Abstract
Cultured cells were established from the hypocotyl of F1 hybrid seedlings of Nicotiana gossei Domin and N. tabacum L. The cultured cells started to die at 26°C, but not at 37°C, which is similar to what occurred in cells of the original hybrid plants. An increase in the number of cells without cytoplasmic strands and acidification of the cytoplasm followed by decomposition of the mitochondria and chloroplasts indicated that vacuolar collapse plays a central role in the execution of cell death. Oxygen but not light was required for cell death. Cellular levels of the superoxide anion and hydrogen peroxide temporarily increased during the early phase at 26°C, while no such oxidative burst was observed at 37°C. The reactive oxygen intermediates are potentially involved in the death of the hybrid cells.
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We thank Dr. Takehiro Masumura of Laboratory of Genetic Engineering, Graduate School of Agriculture, Kyoto Prefectural University for guidance in manipulating the confocal laser scanning microscope.
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Communicated by K.K. Kamo
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Mino, M., Misaka, Y., Ueda, J. et al. Hybrid lethality of cultured cells of an interspecific F1 hybrid of Nicotiana gossei Domin and N. tabacum L.. Plant Cell Rep 24, 179–188 (2005). https://doi.org/10.1007/s00299-005-0923-2
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DOI: https://doi.org/10.1007/s00299-005-0923-2