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Pulsed electromagnetic fields decrease proinflammatory cytokine secretion (IL-1β and TNF-α) on human fibroblast-like cell culture

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Abstract

The clinical use of pulsed electromagnetic fields (PEMF) in osteoarticular pathology is widely extended, although the mechanisms involved are unknown. The aim of this study was to evaluate the action of a new protocol of treatment with PEMF on liquid medium cultures of fibroblast-like cells derivates of mononuclear peripheral blood cells. Fibroblast-like cells growth was obtained in liquid medium culture from mononuclear cells (MNC) of human peripheral blood. The PEMF irradiation protocol included an intensity of 2.25 mT, a frequency of 50 Hz and an application time of 15 min on days 7, 8 and 9 of cell culture. Immunophenotype was performed with specific heterologous monoclonal antibodies for each cell receptor (Vimentin, Cytokeratin, CD34, CD41, CD61 and CD68). The cytokines’ production was determined in the supernatant of the culture medium by means of the Luminex technology. The immunophenotype did not show any statistical difference on comparing treated against non-treated cell cultures on any of the days. In the treatment cell population, the proinflammatory cytokines, IL-1β and TNF-α showed a significant decrease on days 14 and 21 of the culture, whilst IL-10 increased significantly on day 21. It is concluded that PEMF irradiation does not alter the cell immunophenotype of the fibroblast-like cell population, but does provoke a decrease in the production of inflammatory-type cytokines (IL-1β, TNF-α) and an increase in cytokines of lymphocytic origin (IL-10). These facts coincide with the chronology of the clinical effect undergone by patients with osteoarticular pathology after PEMF irradiation.

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Conflict of interest statement

For this work, no financial support was received and the authors have declared no conflicts of interest.

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Authors and Affiliations

  1. Department of Physical Medicine and Rehabilitation, Reina Sofía Hospital, C/Carretera Tarazona, KM 3, CP 31500, Tudela, Spain

    Ignacio Gómez-Ochoa

  2. Department of Animal Pathology, Veterinary Faculty of Zaragoza, C/Miguel Servet, 177, CP 50013, Zaragoza, Spain

    Pablo Gómez-Ochoa

  3. Unit of Haematology, Lozano Blesa Hospital, C/Domingo Miral s/n, CP 50009, Zaragoza, Spain

    Francisco Gómez-Casal & Encarna Cativiela

  4. Department of Inmunology, Lozano Blesa Hospital, C/Domingo Miral s/n, CP 50009, Zaragoza, Spain

    Luis Larrad-Mur

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  1. Ignacio Gómez-Ochoa
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  2. Pablo Gómez-Ochoa
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  3. Francisco Gómez-Casal
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  4. Encarna Cativiela
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Correspondence to Ignacio Gómez-Ochoa.

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Gómez-Ochoa, I., Gómez-Ochoa, P., Gómez-Casal, F. et al. Pulsed electromagnetic fields decrease proinflammatory cytokine secretion (IL-1β and TNF-α) on human fibroblast-like cell culture. Rheumatol Int 31, 1283–1289 (2011). https://doi.org/10.1007/s00296-010-1488-0

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  • DOI: https://doi.org/10.1007/s00296-010-1488-0

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