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Study of the three-way interaction between Trichoderma atroviride, plant and fungal pathogens by using a proteomic approach

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Abstract

The main molecular factors involved in the complex interactions occurring between plants (bean), two different fungal pathogens (Botrytis cinerea, Rhizoctonia solani) and an antagonistic strain of the genus Trichoderma were investigated. Two-dimensional (2-D) electrophoresis was used to analyze separately collected proteomes from each single, two- or three-partner interaction (i.e., plant, pathogenic and antagonistic fungus alone and in all possible combinations). Differential proteins were subjected to mass spectrometry and in silico analysis to search for homologies with known proteins. In the plant proteome, specific pathogenesis-related proteins and other disease-related factors (i.e., potential resistance genes) seem to be associated with the interaction with either one of the two pathogens and/or T. atroviride. This finding is in agreement with the demonstrated ability of Trichoderma spp. to induce systemic resistance against various microbial pathogens. On the other side, many differential proteins obtained from the T. atroviride interaction proteome showed interesting homologies with a fungal hydrophobin, ABC transporters, etc. Virulence factors, like cyclophilins, were up-regulated in the pathogen proteome during the interaction with the plant alone or with the antagonist too. We isolated and confidently identified a large number of protein factors associated to the multi-player interactions examined.

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Acknowledgments

This work was supported by the following projects: FIRB 2002 prot. RBNE01K2E7; PRIN 2003 prot. 2003070719-003, MIUR- PON project No. DD12935 del 02/08/2002; MIUR-PON project No. DD1219 del 05/10/2004; MIUR-PON project No. DD1801 del 31/12/2004; EU TRICHOEST QLK3-2002-02032; EU 2E-BCAs. We also acknowledge the support of G. E. Harman (Cornell University, Geneva, NY, USA) for help on the analysis with the Proteomics Analyzer 4700 MALDI-TOF/TOF.

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Authors and Affiliations

  1. Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Università degli Studi di Napoli Federico II, Via Università 100, 80055, Portici (NA), Italy

    Roberta Marra, Patrizia Ambrosino, Francesco Vinale, Sheridan L. Woo, Rosalia Ciliento, Stefania Lanzuise, Simona Ferraioli, Ida Soriente, Sarah Gigante, David Turrà, Felice Scala & Matteo Lorito

  2. Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell’Alimentazione del CNR, Avellino, Italy

    Virginia Carbone

  3. Istituto CNR per la Protezione delle Piante (IPP), Portici (NA), Italy

    Michelina Ruocco, Felice Scala & Matteo Lorito

  4. Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, Portici (NA), Italy

    Vincenzo Fogliano

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  1. Roberta Marra
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  2. Patrizia Ambrosino
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  3. Virginia Carbone
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  4. Francesco Vinale
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  5. Sheridan L. Woo
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  14. Felice Scala
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  15. Matteo Lorito
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Correspondence to Matteo Lorito.

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Communicated by J. Heitman.

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Marra, R., Ambrosino, P., Carbone, V. et al. Study of the three-way interaction between Trichoderma atroviride, plant and fungal pathogens by using a proteomic approach. Curr Genet 50, 307–321 (2006). https://doi.org/10.1007/s00294-006-0091-0

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