Abstract
An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemical (total acid hydrolysis and methylation analysis) and spectroscopic (FTIR and 1H NMR experiment) analysis. The mannose-rich EPS-1 showed total antioxidant activity (1 mg mL−1 of EPS-I as functional as approximately to 500 ± 5.2 µM of ascorbic acid) and Fe2+ metal ion chelation activity (EC50 = 405.6 µg mL−1) and hydroxyl radical scavenging activity (EC50 = 219.5 µg mL−1). The in vitro cytotoxicity experiment of EPS-I against cervical carcinoma cell line, HeLa cells showed strong cytotoxic effect (LC50 = 267.3 µg mL−1) and at that concentration, it found almost nontoxic against normal healthy cells (HEK-293).
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Acknowledgements
The author Indranil Choudhuri is thankful to CSIR for SRF direct fellowship (File no. 2018/(006)/EMR-I) and DBT BOOST and DST FIST for instrument facility. The author also acknowledges Barun Majumdar for NMR experiment.
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IC, BP, SM, NB designed study. IC has done the experiments, prepared the manuscript. KK helps to collect samples, isolation of strain and partial characterization. PP has done IMViC test. GM helps to perform purification and methylation study. SM helps to analyze NMR, GC–MS data, and prepare the manuscript. NB prepared final manuscript. All the authors read and approved the manuscript.
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Choudhuri, I., Khanra, K., Pariya, P. et al. Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis, and Study on its Antioxidant Activity, and Cytotoxicity Against HeLa Cells. Curr Microbiol 77, 3125–3135 (2020). https://doi.org/10.1007/s00284-020-02130-z
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DOI: https://doi.org/10.1007/s00284-020-02130-z