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Association of Listeria monocytogenes LIPI-1 and LIPI-3 marker llsX with invasiveness

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Abstract

Listeria monocytogenes is an opportunistic pathogen that is widely distributed in the environment. The evolution of its genome has exhibited differences in virulence among strains of the same species. Listeria monocytogenes LIPI-3 (Listeria Pathogenicity Island 3) and LIPI-1 (Listeria Pathogenicity Island 1) are considered responsible for the increased virulence in some strains. The aim of this study was to detect LIPI-1 genes and the llsX gene belonging to LIPI-3 in invasive strains of L. monocytogenes and to establish whether there is a relationship among the invasiveness, presence of the llsX and LIPI-1 genes, and the source of the strains. The results showed that 70% of the strains were invasive, and all these strains except one possessed LIPI-1, which suggests that although there is a correlation between LIPI-1 and invasiveness, the independent mechanisms of LIPI-1 may contribute to invasiveness. In contrast, 35% of the total strains were positive for llsX and were invasive; thus, the results revealed that there is a strong association between llsX and the invasiveness of L. monocytogenes in HEp-2 cells (HeLa contaminant/epithelial in origin). In addition, there is no other association with any other variable in this study. Moreover, the authors found that LIPI-1 and llsX are more frequently found in fresh than in frozen vegetables. Together, the findings provide an approximation for the better understanding of Listeriolysin S (LLS) and its role in the pathogenesis of L. monocytogenes, and a possible relation between virulence factors and food-storage temperature.

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Acknowledgements

REV-R was a recipient of a scholarship from BEIFI-México in 2013–2014. ORR-S is the recipient of COFAA and EDI fellowships. MRE-M is the recipient of SNI and EDI fellowships.

Funding

This work was supported by the SIP-IPN (Grants 20131379, 20141510, 20151345, 20161199, 20170749, 20181268).

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Authors and Affiliations

  1. Laboratorio de Bacteriología Médica, Depto. Microbiología, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional (ENCB-IPN), Prolongación de Carpio y Plan de Ayala, 11340, Mexico City, CDMX, Mexico

    Rodolfo Erik Vilchis-Rangel

  2. Depto. Investigación, Central de Instrumentación de Microscopia, ENCB-IPN, Prolongación de Carpio y Plan de Ayala, 11340, Mexico City, CDMX, Mexico

    María del Rosario Espinoza-Mellado

  3. Laboratorio de Microbiología General, Depto. Microbiología, ENCB-IPN, Prolongación de Carpio y Plan de Ayala, 11340, Mexico City, CDMX, Mexico

    Irving Jesús Salinas-Jaramillo & Oscar Rodolfo Rodas-Suárez

  4. Laboratorio de Recursos Genéticos Microbianos, Centro Nacional de Recursos Genéticos (CNRG), Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias Boulevard de la Biodiversidad 400, Rancho las Cruces, C.P. 47600, Tepatitlán de Morelos, Jalisco, Mexico

    Marcos Daniel Martinez-Peña

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  1. Rodolfo Erik Vilchis-Rangel
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Correspondence to María del Rosario Espinoza-Mellado.

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Vilchis-Rangel, R.E., Espinoza-Mellado, M., Salinas-Jaramillo, I.J. et al. Association of Listeria monocytogenes LIPI-1 and LIPI-3 marker llsX with invasiveness. Curr Microbiol 76, 637–643 (2019). https://doi.org/10.1007/s00284-019-01671-2

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  • DOI: https://doi.org/10.1007/s00284-019-01671-2

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