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Antimicrobial Activity and Biodiversity of Endophytic Fungi in Dendrobium devonianum and Dendrobium thyrsiflorum from Vietman

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Abstract

Endophytic fungi are rich in orchids and have great impacts on their host plants. 53 endophytes (30 isolates from Dendrobium devonianum and 23 endophytic fungi from D. thyrsiflorum) were isolated, respectively, from roots and stems of Dendrobium species. All the fungi were identified by way of morphological and/or molecular biological methods. 30 endophytic fungi in D. devonianum were categorized into 11 taxa and 23 fungal endophytes in D. thyrsiflorum were grouped into 11 genera, respectively. Fusarium was the dominant species of the two Dendrobium species in common. Antimicrobial activity of ethanol extract of fermentation broth of these fungi was explored using agar diffusion test. 10 endophytic fungi in D. devonianum and 11 in D. thyrsiflorum exhibited antimicrobial activity against at least one pathogenic bacterium or fungus among 6 pathogenic microbes (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus). Out of the fungal endophytes isolated from D. devonianum and D. thyrsiflorum, Phoma displayed strong inhibitory activity (inhibition zones in diameter >20 mm) against pathogens. Epicoccum nigrum from D. thyrsiflorum exhibited antibacterial activity even stronger than ampicillin sodium. Fusarium isolated from the two Dendrobium species was effective against the pathogenic bacterial as well as fungal pathogens. The study reinforced the assumption that endophytic fungi isolated from different Dendrobium species could be of potential antibacterial or antifungal resource.

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Acknowledgment

We would like to thank Dr. Youxin Wang for carefully reviewing the manuscript. The research was financially supported by the National Natural Sciences Foundation of China (No. 30830117, 31070300, 30900004) and the National High Technology Research and Development Program of China (No. 2008AA09Z405), and New Drug Preparation Project-Ministry of Science and Technology of China (2009ZX09502-025) and Research of Traditional Chinese Medicine Industry (200807042).

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Authors and Affiliations

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing, 100193, People’s Republic of China

    Yong-Mei Xing, Juan Chen, Jin-Long Cui, Xiao-Mei Chen & Shun-Xing Guo

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  1. Yong-Mei Xing
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  2. Juan Chen
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  3. Jin-Long Cui
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  4. Xiao-Mei Chen
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Correspondence to Shun-Xing Guo.

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Xing, YM., Chen, J., Cui, JL. et al. Antimicrobial Activity and Biodiversity of Endophytic Fungi in Dendrobium devonianum and Dendrobium thyrsiflorum from Vietman. Curr Microbiol 62, 1218–1224 (2011). https://doi.org/10.1007/s00284-010-9848-2

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  • DOI: https://doi.org/10.1007/s00284-010-9848-2

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