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Amplification of Plant Beneficial Microbial Communities During Conversion of Coconut Leaf Substrate to Vermicompost by Eudrilus sp.

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Abstract

The population densities of 15 microbial communities in the coconut leaves + cow manure mixture (10:1 ratio, w/w) and pure cow manure, gut contents of the earthworm, Eudrilus sp., reared on the above substrates and vermicompost produced by the worm were studied. The enumeration was done by dilution plate and most probable number method using several selective and semi-selective microbial media. In the vermicompost produced from coconut leaves + cow manure (CLV) mixture, 9 out of 15 microbial communities, particularly the plant beneficial ones, were amplified whereas five communities were amplified in case of pure cow manure (CMV). The CLV contained significantly high population of fungi, free-living nitrogen fixers, phosphate solubilizers, fluorescent pseudomonads, and silicate solubilizers. The CMV was preponderant with aerobic heterotrophic bacteria, actinomycetes, and Trichoderma spp. Spore formers were present in similar numbers in both the vermicomposts. Presence of Azotobacter was detected only in CMV. The results obtained in this study suggest coconut leaf litter to be a good alternative for cow manure for the production of vermicompost, especially in the areas where coconut is grown in plenty.

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References

  1. Allen ON (1959) Experiments in soil bacteriology, 3rd edn. Burgess, Minneapolis, MN, USA, p 117

    Google Scholar 

  2. Alexander M, Clark FE (1965) Methods for most probable number of Nitrosomonas and Nitrobacter. Methods of soil analysis, Part 2. Soil Sci Soc of America, Madison, USA, pp 1477–1480

    Google Scholar 

  3. Becking JH (1959) Nitrogen fixing bacteria of the genus Beijerinckia in South African soils. Plant Soil 11:193–206

    Article  Google Scholar 

  4. Bunt JS, Rovira AD (1955) Microbiological studies of some subantartic soils. J Soil Sci 6:119–128

    Article  CAS  Google Scholar 

  5. Chet I, Baker R (1981) Isolation and biocontrol potential of Trichoderma hamatum from soil naturally suppressive to Rhizoctonia solani. Phytopathol 71:286–290

    Article  Google Scholar 

  6. Chaoui HI, Zibilske LM, Ohno T (2003) Effects of earthworm casts and compost on soil microbial activity and plant nutrient availability. Soil Biol Biochem 35:295–302

    Article  CAS  Google Scholar 

  7. Decaens T, Rangel AF, Asakawa N, Thomas RJ (1999) Carbon and nitrogen dynamics in ageing earthworm casts in grass lands of the eastern plains of Colombia. Biol Fertil Soils 30:20–25

    Article  CAS  Google Scholar 

  8. Devlee Schanwer D, Lal R (1981) Properties of earthworm casts under secondary tropical forest re-growth. Soil Sci 132:175–181

    Article  Google Scholar 

  9. Drake HL, Horn MA (2007) As the worm turns: the earthworm gut as transient habitat for soil microbial biomes. Ann Rev Microbiol 66:169–189

    Article  CAS  Google Scholar 

  10. Edwards CA, Burrows I (1998) The potential of earthworm compost as plant growth media. In: Edwards CA, Neuhauser E (eds) Earthworm in waste and environmental management. SPB Academic Press, Netherlands, pp 21–32

    Google Scholar 

  11. Edwards CA, Fletcher KE (1988) Interactions between earthworms and microorganisms in organic matter breakdown. Agric Ecosyst Environ 24:235–247

    Article  Google Scholar 

  12. Egert M, Marham S, Wagner B, Scheu S, Friedrich MW (2004) Molecular profiling of 16S rRNA genes reveals diet-related difference of microbial communities, gut, and casts of Lumbricus terrestris L (Oligochaeta: Lumbricidae). FEMS Microbiol Ecol 48:187

    Article  CAS  PubMed  Google Scholar 

  13. Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research, 2nd edn. Wiley and Sons, USA, p 680

    Google Scholar 

  14. Gopal M, Gupta A, Thomas GV (2006) Prospects of using Metarhizium anisopliae to check the breeding of insect pest, Oryctes rhinoceros L., in coconut leaf vermicomposting sites. Biores Technol 97:1801–1806

    Article  CAS  Google Scholar 

  15. Hand P, Hayes WA, Frankland JC, Satchell JE (1988) The vermicomposting of cow slurry. Pedobiologia 31:199–209

    Google Scholar 

  16. Hankin L, Anagnostakis SL (1977) Solid media containing carboxymethyl–cellulose to detect C x cellulase activity of microorganisms. J Gen Microbiol 6:109–115

    Google Scholar 

  17. Hendriksen NB (1991) Gut load and food retention time in the earthworm Lumbricus festivus and L. castaneus: a field study. Biol Fertil Soils 1:170–173

    Article  Google Scholar 

  18. Kale RD, Mallesh BC, Bano K, Bagyaraj DJ (1992) Influence of vermicompost application on the available macronutrients and selected microbial population in a paddy field. Soil Biol Biochem 24:1317–1320

    Article  Google Scholar 

  19. King EO, Ward MN, Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307

    PubMed  CAS  Google Scholar 

  20. Krištǖfek V, Ravasz K, Pižl V (1992) Changes in densities of bacteria and microfungi during gut transit in Lumbricus rubellus and Aporrectodea calignosa (Oligochaeta: Lumbricidae). Soil Biol Biochem 24:1499–1500

    Article  Google Scholar 

  21. Martin JP (1950) Use of acid, rose Bengal and streptomycin in the plate method for estimating soil fungi. Soil Sci 69:215–232

    CAS  Google Scholar 

  22. Parle JN (1963) Microorganisms in the intestines of earthworms. J Gen Microbiol 31:1–11

    Google Scholar 

  23. Parle JN (1963) A microbiological study of earthworm casts. J Gen Microbiol 31:13–22

    CAS  Google Scholar 

  24. Pedersen JC, Hendriksen NB (1993) Effect of passage through the intestinal tract of detrivore earthworms (Lumbricus spp.) on the number of selected gram-negative and total bacteria. Biol Fertil Soils 16:227–232

    Article  Google Scholar 

  25. Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some soil microbial species. Mikrobiologiya 17:362–370

    CAS  Google Scholar 

  26. Prabhu SR, Subramanian P, Bidappa CC, Bopaiah BM (1998) Prospects of improving coconut productivity through vermiculture technologies. Indian Coconut J 29:79–84

    Google Scholar 

  27. Schmidt O, Doube BM, Ryder MH, Killham K (1997) Population dynamics of Pseudomonas corrugata 2140R lux8 in earthworm food and in earthworm casts. Soil Biol Biochem 29:523–528

    Article  CAS  Google Scholar 

  28. Schönholzer F, Hahn D, Zarda B, Zeyer J (2002) Automated image analysis and in situ hybridization as tools to study bacterial populations in food resources, gut and cast of Lumbricus terrestris. Lab J Microbial Methods 48:53–68

    Article  Google Scholar 

  29. Scullion J, Goodacre R, Elliott G, Huang W, Worgan H, Gwynn-Jones D, Griffith G, Darby R, Bailey M, Clegg C, Draper J (2004) Food quality and microbial succession in ageing earthworm casts: standard microbial indices and metabolic fingerprinting. Pedobiologia 47:888–894

    Google Scholar 

  30. Subler S, Edwards CA, Metzger J (1998) Comparing vermicomposts and composts. Biocycle 39:63–66

    CAS  Google Scholar 

  31. Tomati U, Grapelli A, Galli E (1988) The hormone-like effects of earthworm casts on plant growth. Biol Fertil Soils 5:288–294

    Article  CAS  Google Scholar 

  32. Wolter C, Scheu S (1999) Changes in bacterial numbers and hyphal lengths during gut passage through Lumbricus terrestris (Lumbricidae, Oligochaeta). Pedobiologia 43:891–900

    Google Scholar 

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Acknowledgments

The authors are grateful to the anonymous reviewers for their constructive comments on the ms.

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  1. Central Plantation Crops Research Institute, Kasaragod, Kerala, India

    Murali Gopal, Alka Gupta, E. Sunil & George V. Thomas

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  1. Murali Gopal
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  2. Alka Gupta
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  3. E. Sunil
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  4. George V. Thomas
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Correspondence to Murali Gopal.

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Gopal, M., Gupta, A., Sunil, E. et al. Amplification of Plant Beneficial Microbial Communities During Conversion of Coconut Leaf Substrate to Vermicompost by Eudrilus sp.. Curr Microbiol 59, 15–20 (2009). https://doi.org/10.1007/s00284-009-9388-9

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  • DOI: https://doi.org/10.1007/s00284-009-9388-9

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