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The nadA gene of Pseudomonas fluorescens PGPR strain 267.1

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Abstract

An insertion mutant of Pseudomonas fluorescens PGPR strain 267.1 was found to be auxotrophic for niacin (nicotinic acid) and could not synthesize quinolinic acid. The Tn5 interrupted gene was cloned and sequenced. The cloned fragment contained an open reading frame, nadA, capable of encoding a 359-amino-acid protein (39.0 kDa) with substantial identity to various bacterial quinolinate synthetases. The nadA gene complemented quinolinic acid synthesis deficiency and niacin auxotrophy of the P. fluorescens 106P nadA mutant.

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Authors and Affiliations

  1. Department of General Microbiology, University of Maria Curie-Skłodowska, Akademicka 19 st, Lublin, 20-033, Poland

    Monika Marek-Kozaczuk & Anna Skorupska

  2. Department of Biochemistry, University of Maria Curie-Skłodowska, Akademicka 19 st, Lublin, 20-033, Poland

    Jerzy Rogalski

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  1. Monika Marek-Kozaczuk
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  2. Jerzy Rogalski
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  3. Anna Skorupska
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Correspondence to Anna Skorupska.

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Marek-Kozaczuk, M., Rogalski, J. & Skorupska, A. The nadA gene of Pseudomonas fluorescens PGPR strain 267.1. Curr Microbiol 51, 122–126 (2005). https://doi.org/10.1007/s00284-005-4553-2

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  • DOI: https://doi.org/10.1007/s00284-005-4553-2

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