Abstract
The impact of insoluble phosphorus such as aluminum and rock phosphate on alkaline phosphatase activity of polyurethane foam immobilized cyanobacteria was assessed. Polyurethane foam immobilized Nodularia recorded the highest alkaline phosphatase activity of 9.04 (m. mol p-nitrophenol released h–1 mg–1 protein) in vitro. A higher concentration of aluminum phosphate was recorded a 25% reduction in alkaline phosphatase activity, ammonia content, and available phosphorus in culture filtrate of polyurethane foam immobilized cyanobacteria. In general, immobilized cyanobacteria exhibited a higher alkaline phosphatase activity in rock phosphate than aluminum phosphate.
Similar content being viewed by others
Literature Cited
Affolter D, Hall DO (1986) Long term stability of photosynthetic electron transport on polyvinyl foam immobilized cyanobacteria. Photobiochem Photobiophys 11:193–210
Fiske CH, Subba Row Y (1925) Assay of Phosphatase enzyme. J Biol Chem 66:575
Gaftney AM, Markov SA, Gunasekaran M (2001) Utilization of cyanobacteria in photobioreactors for orthophosphate removal from water. Appl Biochem Biotech 91:185–193
Jackson ML (1973) Soil chemical analysis. Prentice Hall of India private limited, New Delhi. p 498
Jensen BB, Raymond P (1983) Effect of oxygen concentration on dark nitrogen fixation and respiration in cyanobacteria. Arch Microbiol 135:287–292
Kannaiyan S, Rao KK, Hall DO (1994) Immobilization of Anabaena azollae from Azolla filculoides in polyvinyl foam for ammonia production in a photobioreactor system. World J Microbiol Biotechnol 24:42–46
Mahesh G, Kannaiyan S (1993) Effect of immobilization of cyanobacteria in solid matrix on ammonia excretion and nitrogen fixing activity. Biotech Lett 15:975–978
Olsen SR, Cole CV, Watanabe FS, Dean LA (1954) Estimation of available phosphorus in soil by extraction with sodium bicarbonate. Washington, DC: US Dept Agric Circ 939. p 24
Richardt W (1971) Catalytic mobilization of phosphate in lake water by Cyanophyta. Hydrobiol 38:377–394
Roychoudhury P, Kaushik BD (1989) Solubilization of Missouri rock phosphate by cyanobacteria. Curr Sci 58:569–570
Shi DJ, Hall DO (1988) The Azolla-Anabaena association. Historical prospective, symbiosis and energy metabolism. Bot Rev 54:353–386
Uma D, Kannaiyan S (1996) Effect of systemic fungicide bavistin the nitrogen status of cyanobacteria under immobilized state in poly urethane foam. S Atr J Bot 62:127–132
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ramalingam, N., Prasanna, B.G. Effect of Aluminum Phosphate on Alkaline Phosphatase Activity of Polyurethane Foam Immobilized Cyanobacteria. Curr Microbiol 53, 194–197 (2006). https://doi.org/10.1007/s00284-005-0443-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00284-005-0443-x