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Isolation and Identification of Natural Endophytic Rhizobia from Rice (Oryza sativa L.) Through rDNA PCR-RFLP and Sequence Analysis

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Abstract

Three novel endophytic rhizobial strains (RRE3, RRE5, and RRE6) were isolated from naturally growing surface-sterilized rice roots. These isolates had the ability to nodulate common bean (Phaseolus vulgaris). Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and sequencing of 16S rDNA of these isolates revealed that RRE3 and RRE5 are phylogenetically very close to Burkholderia cepacia complex, whereas RRE6 has affinity with Rhizobium leguminosarum bv. phaseoli. Plant infection test using gusA reporter gene–tagged construct of these isolates indicated that bacterial cells can go inside and colonize the rice root interiors. A significant increase in biomass and grain yield was also recorded in greenhouse-grown rice plants inoculated with these isolates.

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Acknowledgments

This work is a part of Ph.D. research of Mr. R.P.N. Mishra, which was supported by a BHU research scholarship from the University. The authors are very thankful to Dr. Peter van Berkum, USDA, USA for 16S rDNA sequencing and for providing USDA 2695 strain and to Prof. Barry G. Rolfe, ANU, Australia for providing ANU 843. The authors acknowledge financial support from the Department of Science and Technology, Government of India.

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Author notes

  1. Shree P. Pandey

    Present address: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Stresse 8, Jena, Germany

Authors and Affiliations

  1. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    Ramesh K. Singh, Ravi P. N. Mishra, Hemant K. Jaiswal & Shree P. Pandey

  2. Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    Vinod Kumar

  3. Microbiology Division, Indian Agricultural Research Institute, New Delhi, India

    Sasi B. Rao & Kannepalli Annapurna

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  1. Ramesh K. Singh
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Correspondence to Ramesh K. Singh.

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Singh, R.K., Mishra, R.P., Jaiswal, H.K. et al. Isolation and Identification of Natural Endophytic Rhizobia from Rice (Oryza sativa L.) Through rDNA PCR-RFLP and Sequence Analysis. Curr Microbiol 52, 345–349 (2006). https://doi.org/10.1007/s00284-005-0138-3

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  • DOI: https://doi.org/10.1007/s00284-005-0138-3

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