Abstract
Yeasts are anhydrobiotes that accumulate large amounts of trehalose, which is involved in the vitrification of the cytoplasm during drastic desiccation. The effect of devitrification, which can be induced by the transient exposure of desiccated yeasts to increased humidity or elevated temperature, on the survival of yeast has been studied. A glass transition temperature (Tg)/water activity (aw) diagram of yeast was constructed based on differential scanning calorimetry analysis. The survival rate of yeasts that were equilibrated at different relative humidities (RHs) and temperature values over their Tg range was measured. The results revealed a long period of cell preservation at an intermediate RH (55%), with 100% survival observed after 3 months, a loss of 1.24 log colony-forming units/g recorded after 1 year at 25 °C and full preservation of viability at 75 °C for 60 min and at 100 °C and 12% RH for up to 10 min. These findings led us to conclude that dried yeast can resist low or intermediate RH values and elevated temperatures in the devitrified state. Considering the thermal and humidity fluctuations occurring in the yeast environments, we hypothesized that the supercooled state, which occurs immediately above the Tg after rehydration or heating, is a protective state that is involved in the persistence of yeasts at intermediate humidity levels.
Key points
• Yeast survival for months in a supercooled state is observed at room temperature.
• Dried yeasts survive a 10-min exposure to 100 °C in the supercooled state.
• The supercooled state is suitable for yeast preservation.
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All data generated or analyzed during this study are included in this published article (and its supplementary information files).
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This work was supported by grants from the “National Association of Technical Research (ANRT)”, Phileo by Lesaffre, Lesaffre group and the “Fonds Européen de DEveloppement Régional (FEDER)”.
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PR, LB, and SD conceived and designed the research. PR carried out the experiments. All authors analyzed the data. PR and LB wrote the manuscript and PR, LB, and SD contributed to its critical revision. All authors read and approved the manuscript.
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Ribert, P., Dupont, S., Roudaut, G. et al. Effect of devitrification on the survival and resistance of dried Saccharomyces cerevisiae yeast. Appl Microbiol Biotechnol 105, 6409–6418 (2021). https://doi.org/10.1007/s00253-021-11451-3
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DOI: https://doi.org/10.1007/s00253-021-11451-3