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Optimization of nonribosomal peptides production by a psychrotrophic fungus: Trichoderma velutinum ACR-P1

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Abstract

Trichoderma is an anamorphic filamentous fungal genus with immense potential for production of small valuable secondary metabolites with indispensable biological activities. Microbial dynamics of a psychrotrophic strain Trichoderma velutinum ACR-P1, isolated from unexplored niches of the Shiwalik region, bestowed with rich biodiversity of microflora, was investigated for production of nonribosomal peptides (NRPs) by metabolite profiling by intact-cell mass spectrometry (ICMS) employing matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometer. Being the first report on NRPs production by T. velutinum, studies on optimization of growth conditions by Response Surface Methodology (RSM) for production of NRPs by ACR-P1 was carried out strategically. Multifold enhancement in the yield of NRPs belonging to subfamily SF4 with medium chain of amino acid residues having m/z 1437.9, 1453.9, and 1452.0 at pH 5.9 at 20 °C and of subfamily SF1 with long-chain amino acid residues having m/z 1770.2, 1784.2, 1800.1, 1802.1, and 1815.1 was achieved at pH 7.0 at 25 °C. Complexities of natural mixtures were thus considerably reduced under respective optimized culture conditions accelerating the production of novel microbial natural products by saving time and resources.

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Acknowledgments

The authors acknowledge the funding received from Council of Scientific and Industrial Research (CSIR) under 12th FYP project “Plant-Microbe and Soil Interactions (PMSI).” RS and VPS thank CSIR for providing SRF. This manuscript bears institutional communication number IIIM/1920/2016.

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Authors and Affiliations

  1. Fermentation Technology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India

    Richa Sharma, Farnaz Yusuf & Asha Chaubey

  2. Medicinal Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India

    Varun P. Singh, Deepika Singh & Ram A. Vishwakarma

  3. Academy of Scientific and Innovative Research, New Delhi, 110001, India

    Richa Sharma, Deepika Singh, Ram A. Vishwakarma & Asha Chaubey

  4. Faculty of Sciences, Department of Biotechnology, Shri Mata Vaishno Devi University, Katra, 182320, India

    Varun P. Singh & Anil Kumar

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  1. Richa Sharma
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Correspondence to Deepika Singh or Asha Chaubey.

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Sharma, R., Singh, V.P., Singh, D. et al. Optimization of nonribosomal peptides production by a psychrotrophic fungus: Trichoderma velutinum ACR-P1. Appl Microbiol Biotechnol 100, 9091–9102 (2016). https://doi.org/10.1007/s00253-016-7622-5

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