Abstract
A thermophilic Bacillus coagulans WCP10-4 with tolerance to high concentration of glucose was isolated from soil and used to produce optically pure l-lactic acid from glucose and starch. In batch fermentation at pH 6.0, 240 g/L of glucose was completely consumed giving 210 g/L of l-lactic acid with a yield of 95 % and a productivity of 3.5 g/L/h. In simultaneous saccharification and fermentation at 50 °C without sterilizing the medium, 200 g/L of corn starch was completely consumed producing 202.0 g/L of l-lactic acid. To the best of our knowledge, this strain shows the highest osmotic tolerance to glucose among the strains ever reported for lactic acid production. This is the first report of simultaneous saccharification and fermentation of starch for lactic acid production under a non-sterilized condition.
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References
Bai DM, Wei Q, Yan ZH, Zhao XM, Li XG, Xu SM (2003) Fed-batch fermentation of Lactobacillus lactis for hyper-production of l-lactic acid. Biotechnol Lett 25:1833–1835
Baronofsky JJ, Schreurs WJ, Kashket ER (1984) Uncoupling by acetic acid limits growth of and acetogenesis by Clostridium thermoaceticum. Appl Environ Microbiol 48:1134–1139
Datta R, Henry M (2006) Lactic acid: recent advances in products, processes and technologies—a review. J Chem Technol Biotechnol 81:1119–1129
Ge XY, Yuan J, Qin H, Zhang WG (2011) Improvement of l-lactic acid production by osmotic-tolerant mutant of Lactobacillus casei at high temperature. Appl Microbiol Biotechnol 89:73–78
John RP, Anisha GS, Nampoothiri KM, Pandey A (2009) Direct lactic acid fermentation: focus on simultaneous saccharification and lactic acid production. Biotechnol Adv 27:145–152
Li Z, Ding S, Tan T (2006) l-lactic acid production by Lactobacillus casei fermentation with corn steep liquor-supplemented acid-hydrolysate of soybean meal. Biotechnol J 1:1453–1458
Li Z, Lu J, Zhao L, Xiao K, Tan T (2010) Improvement of L:-lactic acid production under glucose feedback controlled culture by Lactobacillus rhamnosus. Appl Biochem Biotechnol 162:1762–1767
Meng Y, Xue Y, Yu B, Gao C, Ma Y (2012) Efficient production of l-lactic acid with high optical purity by alkaliphilic Bacillus sp. WL-S20. Bioresour Technol 116:334–339
Michelsona T, Kaskb K, Jõgia E, Talpsepa E, Suitsoa I, Nurka A (2006) l(+)-Lactic acid producer Bacillus coagulans SIM-7 DSM 14043 and its comparison with Lactobacillus delbrueckii ssp. lactis DSM 20073. Enzyme Microb Technol 39:861–867
Moon SK, Wee YJ, Choi GW (2012) A novel lactic acid bacterium for the production of high purity l-lactic acid, Lactobacillus paracasei subsp. paracasei CHB2121. J Biosci Bioeng 114:155–159
Narayanan N, Roychoudhury PK, Srivastava A (2004) L (+) lactic acid fermentation and its product polymerization. Electron J Biotechnol 7:165–179
Okano K, Tanaka T, Ogino C, Fukuda H, Kondo A (2010) Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits. Appl Microbiol Biotechnol 85:413–423
Ou MS, Ingram LO, Shanmugam KT (2011) L: (+)-Lactic acid production from non-food carbohydrates by thermotolerant Bacillus coagulans. J Ind Microbiol Biotechnol 38:599–605
Patel MA, Ou MS, Harbrucker R, Aldrich HC, Buszko ML, Ingram LO, Shanmugam KT (2006) Isolation and characterization of acid-tolerant, thermophilic bacteria for effective fermentation of biomass-derived sugars to lactic acid. Appl Environ Microbiol 72:3228–3235
Qin J, Zhao B, Wang X, Wang L, Yu B, Ma Y, Ma C, Tang H, Sun J, Xu P (2009) Non-sterilized fermentative production of polymer-grade l-lactic acid by a newly isolated thermophilic strain Bacillus sp. 2–6. PLoS One 4:e4359
Reddy G, Altaf M, Naveena BJ, Venkateshwar M, Kumar EV (2008) Amylolytic bacterial lactic acid fermentation—a review. Biotechnol Adv 26:22–34
Rosenberg M, Rebros M, Kristofikova L, Malatova K (2005) High temperature lactic acid production by Bacillus coagulans immobilized in LentiKats. Biotechnol Lett 27:1943–1947
Sakai K, Yamanami T (2006) Thermotolerant Bacillus licheniformis TY7 produces optically active l-lactic acid from kitchen refuse under open condition. J Biosci Bioeng 102:132–134
Tashiro Y, Kaneko W, Sun Y, Shibata K, Inokuma K, Zendo T, Sonomoto K (2011) Continuous d-lactic acid production by a novel thermotolerant Lactobacillus delbrueckii subsp. lactis QU 41. Appl Microbiol Biotechnol 89:1741–1750
Walton SL, Bischoff KM, van Heiningen AR, van Walsum GP (2010) Production of lactic acid from hemicellulose extracts by Bacillus coagulans MXL-9. J Ind Microbiol Biotechnol 37:823–830
Wang L, Zhao B, Liu B, Yang C, Yu B, Li Q, Ma C, Xu P, Ma Y (2010a) Efficient production of l-lactic acid from cassava powder by Lactobacillus rhamnosus. Bioresour Technol 101:7895–7901
Wang L, Zhao B, Liu B, Yu B, Ma C, Su F, Hua D, Li Q, Ma Y, Xu P (2010b) Efficient production of l-lactic acid from corncob molasses, a waste by-product in xylitol production, by a newly isolated xylose utilizing Bacillus sp. strain. Bioresour Technol 101:7908–7915
Wang Q, Zhao X, Chamu J, Shanmugam KT (2011) Isolation, characterization and evolution of a new thermophilic Bacillus licheniformis for lactic acid production in mineral salts medium. Bioresour Technol 102:8152–8158
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This research was supported by the Science and Engineering Research Council (SERC) of the Agency for Science, Technology and Research (A*STAR) of Singapore (SERC Grant no 0921590133).
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Zhou, X., Ye, L. & Wu, J.C. Efficient production of l-lactic acid by newly isolated thermophilic Bacillus coagulans WCP10-4 with high glucose tolerance. Appl Microbiol Biotechnol 97, 4309–4314 (2013). https://doi.org/10.1007/s00253-013-4710-7
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DOI: https://doi.org/10.1007/s00253-013-4710-7