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Fluorescence imaging and targeted distribution of bacterial magnetic particles in nude mice

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Abstract

Bacterial magnetic particles (BMPs) are of interest as potential carriers of bioactive macromolecules, drugs, or liposomes. In this study, a high-pressure homogenizer was used to disrupt Magnetospirillum gryphiswaldense strain MSR-1 cells, and BMPs were purified. BMPs were labeled with fluorescence reagent 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocianin perchlorate (DiI) and injected into the tail vein of BALB/c nude mice. Distribution of fluorescence signals of DiI–BMPs in vivo was examined using a whole-body fluorescence imaging system. The result showed that fluorescence signals were detected in liver, stomach, intestine, lungs, and spleen. However, transmission electron microscopy of ultrathin sections indicated that BMPs were mainly present in liver and lungs, but not in the other organs. BMPs could be useful as carriers for targeted drug therapy of diseases of the liver or lung.

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Acknowledgments

We thank Mr. Junzhen Jia and Ms. Haihong Liu (Electron Microscopy Laboratory, China Agricultural University) and Mr. Xun Kuang (Institute of Chemistry, Chinese Academy of Sciences) for the prepared ultrathin sections and TEM and SEM observation. This work was supported by Chinese High Technology Research and Development Program (Grant No. 2007AA021805) and Chinese National Natural Science Foundation (Grant No. 30970041).

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Authors and Affiliations

  1. State Key Laboratory of Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing, 100193, People’s Republic of China

    Tao Tang, Yunping Dai, Fanchao Meng & Ying Li

  2. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medical Center, Peking Union Medical College, Beijing, 100021, People’s Republic of China

    Lianfeng Zhang & Ran Gao

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  1. Tao Tang
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  2. Lianfeng Zhang
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Correspondence to Ying Li.

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Tang, T., Zhang, L., Gao, R. et al. Fluorescence imaging and targeted distribution of bacterial magnetic particles in nude mice. Appl Microbiol Biotechnol 94, 495–503 (2012). https://doi.org/10.1007/s00253-012-3981-8

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  • DOI: https://doi.org/10.1007/s00253-012-3981-8

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