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Transportation mechanism for vanillin uptake through fungal plasma membrane

  • Applied Microbial and Cell Physiology
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Abstract

Protoplasts of the basidiomycete, Fomitopsis palustris (formerly Tyromyces palustris), were utilized to study a function of the fungal plasma membrane. Fungal protoplasts exhibited metabolic activities as seen with intact mycelial cells. Furthermore, the uptake of certain compounds into the protoplast cells was quantitatively observed by using non-radioactive compounds. Vanillin was converted to vanillyl alcohol and vanillic acid as major products and to protocatechuic acid and 1,2,4-trihydroxybenzene as trace products by protoplasts prepared from F. palustris. Extracellular culture medium showed no activity responsible for the redox reactions of vanillin. Only vanillic acid was detected in the intracellular fraction of protoplasts. However, the addition of disulfiram, an aldehyde dehydrogenase inhibitor, caused an intracellular accumulation of vanillin, strongly suggesting that vanillin is taken up by the cell, followed by oxidation to vanillic acid. The addition of carbonylcyanide m-chlorophenylhydrazone, which dissipates the pH gradient across the plasma membrane, inhibited the uptake of either vanillin or vanillic acid into the cell. Thus, the fungus seems to possess transporter devices for both vanillin and vanillic acid for their uptake. Since vanillyl alcohol was only observed extracellularly, the reduction of vanillin was thought to be catalyzed by a membrane system.

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Acknowledgements

This research was supported by a Grant-in-Aid for Scientific Research from the Japan Science Promotion Society (15380224) and by the Science and Technology Incubation Program in Advanced Regions from the Japan Science and Technology Corporation (H.W.).

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  1. Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan

    Motoyuki Shimizu, Yoshinori Kobayashi, Hiroo Tanaka & Hiroyuki Wariishi

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  1. Motoyuki Shimizu
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  2. Yoshinori Kobayashi
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  3. Hiroo Tanaka
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  4. Hiroyuki Wariishi
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Correspondence to Hiroyuki Wariishi.

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Shimizu, M., Kobayashi, Y., Tanaka, H. et al. Transportation mechanism for vanillin uptake through fungal plasma membrane. Appl Microbiol Biotechnol 68, 673–679 (2005). https://doi.org/10.1007/s00253-005-1933-2

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  • DOI: https://doi.org/10.1007/s00253-005-1933-2

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