Abstract
Acetobacter xylinum BPR2001 produces water-insoluble bacterial cellulose (BC). Using a pH sensor for the accurate control of pH, which is one of the most critical factors for efficient BC production, is difficult especially in a baffled shake-flask and an airlift reactor. The buffering capacity of corn steep liquor (CSL) was estimated by measuring β (buffering capacity) values in advance and was used to maintain the pH within the optimal range during the production of BC. When CSL was added to either a shake-flask, a stirred-tank reactor or an airlift reactor, BC production was almost the same as that in cultivations where pH was controlled manually or by a pH sensor.
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Noro, N., Sugano, Y. & Shoda, M. Utilization of the buffering capacity of corn steep liquor in bacterial cellulose production by Acetobacter xylinum . Appl Microbiol Biotechnol 64, 199–205 (2004). https://doi.org/10.1007/s00253-003-1457-6
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DOI: https://doi.org/10.1007/s00253-003-1457-6