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In Situ Dynamics and Spatial Heterogeneity of Soil Bacterial Communities Under Different Crop Residue Management

  • Environmental Microbiology
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Abstract

The effect of the location of wheat residues (soil surface vs. incorporated in soil) on their decomposition and on soil bacterial communities was investigated by the means of a field experiment. Bacterial-automated ribosomal intergenic spacer analysis of DNA extracts from residues, detritusphere (soil adjacent to residues), and bulk soil evidenced that residues constitute the zone of maximal changes in bacterial composition. However, the location of the residues influenced greatly their decomposition and the dynamics of the colonizing bacterial communities. Sequencing of 16S rRNA gene in DNA extracts from the residues at the early, middle, and late stages of degradation confirmed the difference of composition of the bacterial community according to the location. Bacteria belonging to the γ-subgroup of proteobacteria were stimulated when residues were incorporated whereas the α-subgroup was stimulated when residues were left at the soil surface. Moreover, Actinobacteria were more represented when residues were left at the soil surface. According to the ecological attributes of the populations identified, our results suggested that climatic fluctuations at the soil surface select populations harboring enhanced catabolic and/or survival capacities whereas residues characteristics likely constitute the main determinant of the composition of the bacterial community colonizing incorporated residues.

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Acknowledgements

This study was financially supported by the Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME) and the Burgundy region. We would like to thank G. Alavoine, F. Millon, S. Millon, C. Herre, and M.J. Herre for their technical assistance and M. Leleu, responsible for the Estrées-Mons INRA experimental station. Thanks also to Mary Bouley for critical reading of the manuscript.

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  1. Bernard Nicolardot

    Present address: AgroSup Dijon UMR 1210 INRA-AgroSup Dijon–Université de Bourgogne “Biologie et Gestion des Adventices”, 26 Bd Docteur Petitjean, BP 87999, 21079, Dijon Cedex, France

Authors and Affiliations

  1. UMR Microbiologie du Sol et de l’Environnement, INRA/Université de Bourgogne CMSE, BP 86510, 17 rue de Sully, 21065, Dijon Cedex, France

    Noémie Pascault, Fabiola Bastian, Lionel Ranjard & Pierre-Alain Maron

  2. INRA-Unité d’agronomie de Laon-Reims-Mons, 2 Esplanade Roland Garros, BP 224, 51686, Reims Cedex 2, France

    Bernard Nicolardot

  3. INRA, UMR-614 Fractionnement des Agro-Ressources et Environnement, 2 Esplanade Roland Garros, BP 224, 51686, Reims Cedex 2, France

    Pascal Thiébeau

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  1. Noémie Pascault
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Correspondence to Pierre-Alain Maron.

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Pascault, N., Nicolardot, B., Bastian, F. et al. In Situ Dynamics and Spatial Heterogeneity of Soil Bacterial Communities Under Different Crop Residue Management. Microb Ecol 60, 291–303 (2010). https://doi.org/10.1007/s00248-010-9648-z

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