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Population Dynamics and Identification of Endophytic Bacteria Antagonistic Toward Plant-Pathogenic Fungi in Cotton Root

  • Plant Microbe Interactions
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Abstract

The antagonistic potentials of endophytic bacteria isolated from the roots of six cotton cultivars at different developmental stages were determined in vitro toward three pathogens: Verticillium dahliae Kleb V107 and V396 and Fusarium oxysporum f.sp. vasinfectum (F108). The populations of antagonistic endophytic bacteria (AEB) toward V107, V396, and F108 at the flowering and maturation stages were significantly higher than those at the seedling stage were. More AEB were found to be antagonistic toward pathogens V396 and F108 than V107. Results from the multivariate analysis of variance showed that the populations of AEB were significantly different for the main factors of cultivars, stages, and their interactions. Based on 16S rDNA sequence analysis, the 39 AEB isolates that antagonized V107, V396, and F108 (BAEB) consisted of seven genera, in which the genus of Enterobacter (17 out of 39) and Pantoea (14 out of 39) were predominant among the BAEB isolates. Characterized by BOX-PCR fingerprints, these 39 BAEB isolates represented 35 different cluster types. To explore the antagonistic mechanisms, the agar diffusion method was used to detect cell-wall-degrading enzyme activity and siderophore secretion. Nearly half of these BAEB isolates showed protease and chitinase activity, while all 39 BAEB isolates excreted siderophores. However, pectinase, cellulase, and xylanase activity were hardly detected. A germination experiment revealed that nine of the 39 BAEB isolates significantly improved the vigor index of the cotton seedlings.

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Acknowledgments

We thank Haiwei Lü, Qiaojie Wei, and Cuan Feng for their assistance isolating and identifying cotton endophytic bacteria and Changhua Shang for his guidance in the cluster analysis of the BOX fingerprints of BAEB.

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Authors and Affiliations

  1. College of Agronomy, National Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China

    Chun-Hong Li & Can-Ming Tang

  2. College of Life Sciences, Nanjing Agricultural University Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing, 210095, Jiangsu, People’s Republic of China

    Ming-Wen Zhao & Shun-Peng Li

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  1. Chun-Hong Li
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Correspondence to Ming-Wen Zhao or Can-Ming Tang.

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Li, CH., Zhao, MW., Tang, CM. et al. Population Dynamics and Identification of Endophytic Bacteria Antagonistic Toward Plant-Pathogenic Fungi in Cotton Root. Microb Ecol 59, 344–356 (2010). https://doi.org/10.1007/s00248-009-9570-4

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