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Diversity of Culturable Bacteria Isolated from Root Domains of Moso Bamboo (Phyllostachys edulis)

  • Plant Microbe Interactions
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Abstract

The distribution of culturable bacteria in the rhizosphere, rhizoplane, and interior root tissues of moso bamboo plants was investigated in this study. Of the 182 isolates showing different colony characteristics on Luria–Bertani and King B plates, 56 operational taxonomic units of 22 genera were identified by 16S ribosomal RNA gene sequence analysis. The majority of root endophytic bacteria were Proteobacteria (67.5%), while the majority of rhizospheric and rhizoplane bacteria were Firmicutes (66.3% and 70.4%, respectively). The most common genus in both the rhizosphere and on the rhizoplane was Bacillus (42.4% and 44.4%, respectively), while Burkholderia was the most common genus inside the roots, comprising 35.0% of the isolates from this root domain. The endophytic bacterial community was less diverse than the rhizoplane and rhizospheric bacterial communities. Members of Lysinibacillus, Bacillus, and Burkholderia were found in all three root domains, whereas many isolates were found in only a single domain. Our results show that the population diversity of culturable bacteria is abundant in the root domains of moso bamboo plants and that obvious differences exist among the rhizospheric, rhizoplane, and endophytic bacterial communities.

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Acknowledgements

We thank Professor Xiaoping Gu and Dr. Jinjun Yue from The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, for assistance with moso bamboo plant samples collection. This work was supported by the project of the Forest Scientific and Technical Supporting Programs (2006BAD01A18) and Postdoctoral Seed Fund Program of Hebei University, People’s Republic of China.

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Authors and Affiliations

  1. Research Center of Biological Engineering for Hebei Province, College of life Sciences, Hebei University, Baoding, 071002, China

    Jigang Han, Dongliang Xia, Lei Sun & Liping Zhang

  2. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Haidian District, Beijing, 100091, China

    Dongliang Xia & Lubin Li

  3. Beijing Key Laboratory of New Technology in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, China

    Kai Yang

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  1. Jigang Han
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Correspondence to Jigang Han or Lubin Li.

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Han, J., Xia, D., Li, L. et al. Diversity of Culturable Bacteria Isolated from Root Domains of Moso Bamboo (Phyllostachys edulis). Microb Ecol 58, 363–373 (2009). https://doi.org/10.1007/s00248-009-9491-2

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