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Decomposition of Cr(V)-diols to Cr(III) Complexes by Arthrobacter oxydans

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Abstract

We demonstrated previously that Cr(VI) is readily reduced to oxoCr(V)-diols at the surface of Arthrobacter oxydans—a Gram-positive aerobic bacteria isolated from Columbia basalt rocks originated from a highly contaminated site in the USA. Here, we report an electron spin resonance (ESR) study of Cr(III) hydroxide formation from Cr(V)-diols by this bacterial strain as cells were exposed to 35, 200, and 400 mg/L of Cr(VI) under aerobic conditions as a batch culture and as lyophilized cells. The time-dependent ESR measurements show that the half-time of Cr(III) formation is almost equal to that of Cr(V) decomposition, which is in the range of 3–6 days for all cases. This rate is at least 300 times slower than that of Cr(V) formation. Additionally, atomic absorption spectrometry was also employed to examine the time course of total chromium in bacterial cells. This is the first time the kinetics of Cr(III) complexes formation in bacteria is evaluated.

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Acknowledgments

This work was funded by Grant #STCU-GNSF 4330/131 from the Ukrainian Science and Technology Centre (STCU) and Georgian National Science Foundation (GNSF). The participation at the BioMicroWorld2007 was financed by the GNSF Travel Grant #TR07/82. We gratefully acknowledge Prof. D. Pataraya for providing bacterial samples and Miss L. Asanishvili for her assistance during AAS experiments.

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Authors and Affiliations

  1. Andronikashvili Institute of Physics, Tbilisi, 0177, Georgia

    Nelly Y. Tsibakhashvili, Tamaz L. Kalabegishvili & Alexander N. Rcheulishvili

  2. Chavchavadze State University, Tbilisi, 0179, Georgia

    Nelly Y. Tsibakhashvili, Tamaz L. Kalabegishvili, Ivane G. Murusidze, Olia A. Rcheulishvili & Salome M. Kerkenjia

  3. Center for Environmental Biotechnology, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA

    Hoi-Ying N. Holman

  4. Laboratory of Environmental Biotechnology, Andronikashvili Institute of Physics, 6, Tamarashvili Str., Tbilisi, 0177, Georgia

    Nelly Y. Tsibakhashvili

Authors
  1. Nelly Y. Tsibakhashvili
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  2. Tamaz L. Kalabegishvili
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  3. Alexander N. Rcheulishvili
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  4. Ivane G. Murusidze
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  5. Olia A. Rcheulishvili
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  6. Salome M. Kerkenjia
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  7. Hoi-Ying N. Holman
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Correspondence to Nelly Y. Tsibakhashvili.

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Tsibakhashvili, N.Y., Kalabegishvili, T.L., Rcheulishvili, A.N. et al. Decomposition of Cr(V)-diols to Cr(III) Complexes by Arthrobacter oxydans . Microb Ecol 57, 360–366 (2009). https://doi.org/10.1007/s00248-008-9476-6

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  • DOI: https://doi.org/10.1007/s00248-008-9476-6

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