Abstract
Recently, the human protein responsible for replicative mtDNA helicase activity was identified and designated Twinkle. Twinkle has been implicated in autosomal dominant progressive external ophthalmoplegia (adPEO), a mitochondrial disorder characterized by mtDNA deletions. The Twinkle protein appears to have evolved from an ancestor shared with the bifunctional primase-helicase found in the T-odd bacteriophages. However, the question has been raised as to whether human Twinkle possesses primase activity, due to amino acid sequence divergence and absence of a zinc-finger motif thought to play an integral role in DNA binding. To date, a primase protein participating in mtDNA replication has not been identified in any eukaryote. Here we investigate the wider phylogenetic distribution of Twinkle by surveying and analyzing data from ongoing EST and genome sequencing projects. We identify Twinkle homologues in representatives from five of six major eukaryotic assemblages (“supergroups”) and present the sequence of the complete Twinkle gene from two members of Amoebozoa, a supergroup of amoeboid protists at the base of the opisthokont (fungal/metazoan) radiation. Notably, we identify conserved primase motifs including the zinc finger in all Twinkle sequences outside of Metazoa. Accordingly, we propose that Twinkle likely serves as the primase as well as the helicase for mtDNA replication in most eukaryotes whose genome encodes it, with the exception of Metazoa.
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Acknowledgments
Twinkle sequences generated under the auspices of the Protist EST Program were generously provided by R.W. Lee, Dalhousie University (P. wickerhamii), and B.F. Lang, Université de Montréal (J. bahamiensis, M. jakobiformis, S. ecuadoriensis). Preliminary sequence data for Tetrahymena thermophila were obtained from The Institute for Genomic Research (TIGR) web site, at http://www.tigr.org. We are grateful to one of the reviewers of this manuscript for pointing out the Twinkle-like helicase sequence in the recently released Rhizopus oryzae genome data. We thank M. Dlutek for performing DNA sequencing. This work was supported by funding to M.W.G. from the Canadian Institutes of Health Research (Operating Grant MOP-4355) and Genome Canada through Genome Atlantic and the Atlantic Innovation Fund (Protist EST Program). M.W.G. is pleased to acknowledge salary support from the Canada Research Chairs Program and the Canadian Institute for Advanced Research.
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Shutt, T.E., Gray, M.W. Twinkle, the Mitochondrial Replicative DNA Helicase, Is Widespread in the Eukaryotic Radiation and May Also Be the Mitochondrial DNA Primase in Most Eukaryotes. J Mol Evol 62, 588–599 (2006). https://doi.org/10.1007/s00239-005-0162-8
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DOI: https://doi.org/10.1007/s00239-005-0162-8