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Kinetics of Influenza Virus Fusion with the Endosomal and Plasma Membranes of Cultured Cells. Effect of Temperature

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Abstract

We performed a detailed kinetic analysis of influenza virus fusion with the endosomal and plasma membranes of Madin Darby canine kidney (MDCK) cells and provided a comparison of the kinetic parameters obtained for both cases at 20°C and 37°C. Using our mass action kinetic model, we determined that the fusion rate constant, f, for influenza virus with the endosomal membrane was 0.02 s−1 at 37°C and 0.0035 s−1 at 20°C. The analysis of the fusion kinetics of influenza virus with the plasma membrane yielded that the fusion rate constants were close to those deduced with the endosomal membrane. The systematic kinetic analysis performed in this study provides for the first time a biophysical support for studies on influenza virus-cell fusion where the acidic endosomal internal environment is simulated artificially by lowering the pH of the medium.

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Acknowledgements

This work was supported by Grant POCTI/CVT/42700/2001 from FCT, Portugal (I. N.-C.), by European Union Grant BIO4-CT97-2191 (M. C. P. L.), and by Grant PRAXIS/PCNA/P/BIO/45/96 from JNICT, Portugal (M. C. P. L.).

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Authors and Affiliations

  1. Department of Biochemistry, University of Coimbra, Apartado 3126, 3000 Coimbra, Portugal

    I. Nunes-Correia & M. C. Pedroso de Lima

  2. Center for Neuroscience and Cell Biology, University of Coimbra, 3000 Coimbra, Portugal

    I. Nunes-Correia & M. C. Pedroso de Lima

  3. Seagram Center for Soil and Water Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel

    S. Nir

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  1. I. Nunes-Correia
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Correspondence to M. C. Pedroso de Lima.

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Nunes-Correia, I., Nir, S. & Pedroso de Lima, M. Kinetics of Influenza Virus Fusion with the Endosomal and Plasma Membranes of Cultured Cells. Effect of Temperature . J. Membrane Biol. 195, 21–26 (2003). https://doi.org/10.1007/s00232-003-2040-x

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  • DOI: https://doi.org/10.1007/s00232-003-2040-x

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