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Manual stimulation of forearm muscles does not improve recovery of motor function after injury to a mixed peripheral nerve

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Abstract

Transection and re-anastomosis of the purely motor facial nerve leads to poor functional recovery. However, we have recently shown in rat that manual stimulation (MS) of denervated vibrissal muscles reduces the number of polyinnervated motor endplates and promotes full recovery of whisking. Here, we examined whether MS of denervated rat forearm muscles would also improve recovery following transection and suture of the mixed (sensory and motor) median nerve (median–median anastomosis, MMA). Following MMA of the right median nerve, animals received no postoperative treatment, daily MS of the forearm muscles or handling only. An almost identical level of functional recovery, measured by the force of grip in grams, was reached in all animals by the sixth postoperative week and maintained till 3 months following surgery regardless of the postoperative treatment. Also, we found no differences among the groups in the degree of axonal sprouting, the extent of motor endplate polyinnervation and in the soma size of regenerated motoneurons. Taken together, we show that while MS is beneficial following motor nerve injury, combined strategies will be required for functional recovery following mixed nerve injury.

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Acknowledgments

The present study was supported by the Jean-Uhrmacher Foundation, Köln Fortune Programm, and the DFG (AN 331/3-1, AN 331/5-1). SAD is a Senior Research Fellow (National Health & Medical Research Council, Australia; grant ID: 254670). The skillful assistance of D. Bösel, D. Felder, N. Lange, C. Backhausen and L. Wilken is highly appreciated.

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Authors and Affiliations

  1. Department of Hand-, Plastic-, and Reconstructive Surgery with Burn Unit, BG-Trauma Centre, University of Tuebingen, Tuebingen, Germany

    N. Sinis & H. E. Schaller

  2. Department of Otorhinolaryngology, Friedrich-Schiller University Jena, 07740, Jena, Germany

    O. Guntinas-Lichius

  3. Centre for Molecular Neurobiology, University of Hamburg, Hamburg, Germany

    A. Irintchev

  4. Department of Trauma, Hand and Reconstructive Surgery, University of Cologne, Cologne, Germany

    E. Skouras

  5. Institut für Anatomie der Universität zu Köln, Joseph-Stelzmann-Strasse 9, 50924, Cologne, Germany

    S. Kuerten & D. N. Angelov

  6. Department of Anatomy, Histology and Embryology, Medical University Varna, Varna, Bulgaria

    S. P. Pavlov

  7. School of Animal Biology and Western Australian Institute for Medical Research, The University of Western Australia, Perth, WA, Australia

    S. A. Dunlop

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  1. N. Sinis
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  9. D. N. Angelov
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S. A. Dunlop and D. N. Angelov contributed equally and share last authorship.

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Sinis, N., Guntinas-Lichius, O., Irintchev, A. et al. Manual stimulation of forearm muscles does not improve recovery of motor function after injury to a mixed peripheral nerve. Exp Brain Res 185, 469–483 (2008). https://doi.org/10.1007/s00221-007-1174-y

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