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Identification of c-Fos immunoreactive brainstem neurons activated during fictive mastication in the rabbit

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Abstract

In the present study we used the expression of the c-Fos-like protein as a “functional marker” to map populations of brainstem neurons involved in the generation of mastication. Experiments were conducted on urethane-anesthetized and paralyzed rabbits. In five animals (experimental group), rhythmical bouts of fictive masticatory-like motoneuron activity (cumulative duration 60–130 min) were induced by electrical stimulation of the left cortical “masticatory area” and recorded from the right digastric motoneuron pool. A control group of five animals (non-masticatory) were treated in the same way as the experimental animals with regard to surgical procedures, anesthesia, paralysis, and survival time. To detect the c-Fos-like protein, the animals were perfused, and the brainstems were cryosectioned and processed immunocytochemically. In the experimental group, the number of c-Fos-like immunoreactive neurons increased significantly in several brainstem areas. In rostral and lateral areas, increments occurred bilaterally in the borderzones surrounding the trigeminal motor nucleus (Regio h); the rostrodorsomedial half of the trigeminal main sensory nucleus; subnucleus oralis-γ of the spinal trigeminal tract; nuclei reticularis parvocellularis pars α and nucleus reticularis pontis caudalis (RPc) pars α. Further caudally-enhanced labeling occurred bilaterally in nucleus reticularis parvocellularis and nucleus reticularis gigantocellularis (Rgc) including its pars-α. Our results provide a detailed anatomical record of neuronal populations that are correlated with the generation of the masticatory motor behavior.

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Fig. 1
Fig. 2A–B
Fig. 3A–D

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Abbreviations

Alv inf:

Inferior alveolar nerve

c-Fos-like-IR:

c-Fos-like immunoreactivity

Coe:

Nucleus coeruleus

DAB:

3,3′-diaminobenzidine

Dig:

Digastric nerve

HG:

Central gray matter (Höhlengrau)

k:

Cell group k

NVmt:

Trigeminal motor nucleus

NVmt-dig:

Digastric motoneuron subnucleus of the trigeminal motor nucleus

NVsnpr:

Main sensory trigeminal nucleus

NVspo-α:

Subnucleus-α of the oral nucleus of the spinal trigeminal tract

NVspo-β:

Subnucleus-β of the oral nucleus of the spinal trigeminal tract

NVspo-γ:

Subnucleus-γ of the oral nucleus of the spinal trigeminal tract

NintV:

Intertrigeminal subnucleus

NsV:

Supratrigeminal subnucleus

NrVII:

Nucleus retrofacialis

Ntsl:

Nucleus tractus solitarii

NVII:

Facial motor nucleus

NXII:

Hypoglossal motor nucleus

Ols:

Nucleus olivaris superior

Ol pr:

Nucleus olivaris principalis

PBS:

Phosphate buffered saline

p Ols:

Nucleus paraolivaris superior

Prph:

Nucleus praepositus hypoglossi

Pyr:

Tractus pyramidalis

Ramg:

Nucleus raphes magnus

Regio h:

Borderzone surrounding the trigeminal motor nucleus

Rl:

Nucleus reticularis lateralis

Rgc:

Nucleus reticularis gigantocellularis

Rgc-α:

Pars α of nucleus reticularis gigantocellularis

RPc:

Nucleus reticularis pontis caudalis

RPc-α:

Pars α of nucleus reticularis pontis caudalis

Rpc:

Nucleus reticularis parvocellularis

Rpc-α:

Pars α of nuclei reticularis parvocellularis

Sg:

Nucleus supragenualis

Trg:

Nucleus triangularis

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Acknowledgements

This work was supported by a grant from the Swedish MRC (04X-00045) and the Canadian Institutes of Health Research (Grant No. MT14392), Västerbottens läns landsting and the Medical and Odontological Faculty, Umeå University, Sweden. We would like to thank Erland Danielsson, Gunvor Hellström, Tommy Olsson, Jan Stenman and Lev Novikov for their support and help. We also thank Kristina Olsson-Gibbs for stylistic revision.

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Authors and Affiliations

  1. Department of Integrative Medical Biology, Section for Physiology, Umeå University, 901 87, Umeå, Sweden

    T. Athanassiadis, K.Å. Olsson & K-G. Westberg

  2. Centre de Recherche en Sciences Neurologiques, Université de Montréal, Montréal, QC, Canada, H3C 3J7

    A. Kolta

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  1. T. Athanassiadis
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  2. K.Å. Olsson
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  3. A. Kolta
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  4. K-G. Westberg
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Correspondence to K-G. Westberg.

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Athanassiadis, T., Olsson, K., Kolta, A. et al. Identification of c-Fos immunoreactive brainstem neurons activated during fictive mastication in the rabbit. Exp Brain Res 165, 478–489 (2005). https://doi.org/10.1007/s00221-005-2319-5

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  • DOI: https://doi.org/10.1007/s00221-005-2319-5

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