Abstract
Nanostructured lipid carriers (NLC) could be used as suitable carriers of water-insoluble nutrients such as coenzyme Q10 (CoQ10) improve their bioavailability. The CoQ10-NLC was prepared by high-pressure homogenization method. The freshly prepared CoQ10-NLC showed ellipsoid morphology under atomic force microscope, and the average encapsulation efficiency was about 98.4 ± 0.3%. Good physical and chemical stability was observed by photon correlation spectrum and HPLC, respectively. In 3 months’ storage, the concentration of CoQ10-NLC kept at 8.5 ± 1.5 mg/mL; the particle size was 190 ± 30 nm with polydispersity index (PDI) lower than 0.1. Lyophilization was proved feasible for the storage of NLC dispersion. The formulation also showed the potent possibility of application on beverage. Particle size remained relatively steady in simulated beverage solution for 3 months. The oral bioavailability study was carried out using Wistar rats. The relative bioavailability of CoQ-NLC was about 200% compared with CoQ10 suspension. In conclusion, the NLC formulation showed good stability and improved bioavailability. It demonstrated a promising perspective for daily consuming and industrial production.
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References
Müller RH, Radtke M, Wissing SA (2002) Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Adv Drug Deliv Rev 54(Supplement (0)):S131–S155. doi:10.1016/s0169-409x(02)00118-7
Zhuang CY, Li N, Wang M, Zhang XN, Pan WS, Peng JJ, Pan YS, Tang X (2010) Preparation and characterization of vinpocetine loaded nanostructured lipid carriers (NLC) for improved oral bioavailability. Int J Pharm 394(1–2):179–185. doi:10.1016/j.ijpharm.2010.05.005
Junyaprasert VB, Teeranachaideekul V, Souto EB, Boonme P, Muller RH (2009) Q(10)-loaded NLC versus nanoemulsions: stability, rheology and in vitro skin permeation. Int J Pharm 377(1–2):207–214. doi:10.1016/j.ijpharm.2009.05.020
Sandoval B (2009) Perspectives on FDA’s regulation of nanotechnology: emerging challenges and potential solutions. Compr Rev Food Sci Food Saf 8(4):375–393. doi:10.1111/j.1541-4337.2009.00088.x
Langsjoen PH, Langsjoen AM (1999) Overview of the use of CoQ(10) in cardiovascular disease. Biofactors 9(2–4):273–284
Terao K, Nakata D, Fukumi H, Schmid G, Arima H, Hirayama F, Uekama K (2006) Enhancement of oral bioavailability of coenzyme Q(10) by complexation with gamma-cyclodextrin in healthy adults. Nutr Res 26(10):503–508. doi:10.1016/j.nutres.2006.08.004
Balakrishnan P, Lee BJ, Oh DH, Kim JO, Lee YI, Kim DD, Jee JP, Lee YB, Woo JS, Yong CS, Choi HG (2009) Enhanced oral bioavailability of coenzyme Q(10) by self-emulsifying drug delivery systems. Int J Pharm 374(1–2):66–72. doi:10.1016/j.ijpharm.2009.03.008
Ozaki A, Muromachi A, Sumi M, Sakai Y, Morishita K, Okamoto T (2010) Emulsification of coenzyme q(10) using gum arabic increases bioavailability in rats and human and improves food-processing suitability. J Nutr Sci Vitaminol 56(1):41–47
Müller RH, Maassen S, Schwarz C, Mehnert W (1997) Solid lipid nanoparticles (SLN) as potential carrier for human use: interaction with human granulocytes. J Controlled Releas 47(3):261–269. doi:10.1016/s0168-3659(97)01653-2
Hsu C-H, Cui Z, Mumper R, Jay M (2003) Preparation and characterization of novel coenzyme Q10 nanoparticles engineered from microemulsion precursors. AAPS Pharm Sci Tech 4(3):24–35. doi:10.1208/pt040332
Tang JG, Xia Q, Liu GY (2010) Storage stability of alpha-lipoic acid-loaded lipid nanoparticles. Chin J Process Eng 10(02):6
Müller RH, Mäder K, Gohla S (2000) Solid lipid nanoparticles (SLN) for controlled drug delivery–a review of the state of the art. Eur J Pharm Biopharm 50(1):161–177. doi:10.1016/s0939-6411(00)00087-4
Constantinides PP, Scalart J-P, Lancaster C, Marcello J, Marks G, Ellens H, Smith PL (1994) Formulation and intestinal absorption enhancement evaluation of water-in-oil microemulsions incorporating medium-chain glycerides. Pharm Res 11(10):1385–1390. doi:10.1023/a:1018927402875
Constantinides PP, Welzel G, Ellens H, Smith PL, Sturgis S, Yiv SH, Owen AB (1996) Water-in-oil microemulsions containing medium-chain fatty acids/salts: formulation and intestinal absorption enhancement evaluation. Pharm Res 13(2):210–215. doi:10.1023/a:1016030812272
Paliwal R, Rai S, Vaidya B, Khatri K, Goyal AK, Mishra N, Mehta A, Vyas SP (2009) Effect of lipid core material on characteristics of solid lipid nanoparticles designed for oral lymphatic delivery. Nanomed Nanotechnol Biol Med 5(2):184–191. doi:10.1016/j.nano.2008.08.003
Neslihan Gursoy R, Benita S (2004) Self-emulsifying drug delivery systems (SEDDS) for improved oral delivery of lipophilic drugs. Biomed Pharmacother 58(3):173–182. doi:10.1016/j.biopha.2004.02.001
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Financial support from International Cooperation Project (Grant No. 2008DFB50060).
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Liu, G.Y., Wang, J.M. & Xia, Q. Application of nanostructured lipid carrier in food for the improved bioavailability. Eur Food Res Technol 234, 391–398 (2012). https://doi.org/10.1007/s00217-011-1645-z
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DOI: https://doi.org/10.1007/s00217-011-1645-z