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A fluorescent artificial receptor with specific imprinted cavities to selectively detect colistin

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Abstract

A novel and facile fluorescent artificial receptor on the basis of the molecularly imprinted polymer-coated graphene quantum dots was engineered successfully to detect colistin. The colistin imprinted graphene quantum dots (CMIP-GQDs) was synthesized by vinyl-based radical polymerization between functional monomers and crosslinker at around the template molecule on the surface of graphene quantum dots. The size of bare, CNIP-GQDs, and CMIP-GQDs was about 4.8 ± 0.6 nm, 18.4 ± 1.7 nm, and 19.7 ± 1.3 nm, respectively. The CMIP-GQDs, which showed the strong fluorescence emission at 440 nm with the excitation wavelength fixed at 380 nm, had excellent selectivity and specificity to rapidly recognize and detect colistin. The linear range of fluorescence quenching of this fluorescent artificial receptor for detection colistin was 0.016–2.0 μg mL−1 with a correlation coefficient (R2) of 0.99919, and the detection limit was 7.3 ng mL−1 in human serum samples. The designed receptor was successfully applied to detect colistin in human serum samples and it achieved excellent recoveries shifted from 93.8 to 105%.

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Authors and Affiliations

  1. Faculty of Pharmacy, Department of Basic Sciences, Ankara Medipol University, 06050, Ankara, Turkey

    Eylem Turan

  2. Faculty of Engineering, Department of Chemical Engineering, Van Yuzuncu Yil University, 65080, Van, Turkey

    Adem Zengin

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  1. Eylem Turan
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Correspondence to Eylem Turan.

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All procedures performed in studies involving human participants were approved by Istanbul Medipol University Non-invasive Clinical Research Ethics Committee, Turkey, and in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Turan, E., Zengin, A. A fluorescent artificial receptor with specific imprinted cavities to selectively detect colistin. Anal Bioanal Chem 412, 7417–7428 (2020). https://doi.org/10.1007/s00216-020-02873-5

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