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Dual-mode immunoassay based on shape code and infrared absorption fingerprint signals of silica nanorods

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Abstract

Silica nanorods were synthesized through a simple one-pot emulsion-droplet-based growth method, in which tetraethylorthosilicate (TEOS) was used as the silica source, ammonia as the catalyst, and polyvinylpyrrolidone (PVP) as the structure-directing agent and stabilizer. By controlling hydrolysis and condensation in the reaction process, we regulated the aspect ratios and the infrared (IR) absorption fingerprint signals (the transverse optical and the longitudinal optical phonon modes) of the silica nanorods. Based on this, a dual-mode immunoassay was performed for detecting model target analyte, human IgG. The shape code of the silica nanorods was used for simple, rapid qualitative, and sensitive semi-quantitative immunoassay by using a conventional optical microscope. The characteristic IR absorption fingerprint signals of the silica nanorods allowed for reliable quantitative immunoassay with good selectivity and high specificity. The detection limit and the linear range were found out to be 0.5 pM and 1 pM–10 nM, respectively. We expect that such dual-mode immunoassay could be applied for the detection of other analytes, such as protein, nucleic acids, bacteria, viruses, explosives, toxins, and so on.

A simple dual-mode immunoassay was performed using the shape code and infrared absorption fingerprint signals of silica nanorods as detection signals.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant Nos. 51272040, 11604043, and 51372096), the 111 project (No. B13013), and Program for New Century Excellent Talents in University of Ministry of Education of China (Grant No. NCET-12-0815).

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Authors and Affiliations

  1. Key Laboratory of UV-Emitting Materials and Technology, Northeast Normal University, Ministry of Education, Changchun, Jilin, 130024, China

    Pengfei Zhao, Ran Ni, Kexin Wang, Xia Hong, Yadan Ding, Tie Cong & Junping Liu

  2. Department of Basic Medicine, Gerontology Department of First Bethune Hospital, University of Jilin, Changchun, Jilin, 130021, China

    Huiying Zhao

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  1. Pengfei Zhao
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  2. Ran Ni
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  3. Kexin Wang
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  4. Xia Hong
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  5. Yadan Ding
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  6. Tie Cong
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  7. Junping Liu
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  8. Huiying Zhao
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Correspondence to Xia Hong or Huiying Zhao.

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Zhao, P., Ni, R., Wang, K. et al. Dual-mode immunoassay based on shape code and infrared absorption fingerprint signals of silica nanorods. Anal Bioanal Chem 409, 4207–4213 (2017). https://doi.org/10.1007/s00216-017-0369-8

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  • DOI: https://doi.org/10.1007/s00216-017-0369-8

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