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Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

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Abstract

This review focuses on recent advances in the development of functionalizable antifouling coatings and their applications in label-free optical biosensors. Approaches to the development of antifouling coatings, ranging from self-assembled monolayers and PEG derivatives to ultra-low-fouling polymer brushes, are reviewed. Methods of preparation and characterization of antifouling coatings and the functionalization of antifouling coatings with bioreceptors are reviewed, and the effect of functionalization on the fouling properties of biofunctional coating is discussed. Special attention is given to biofunctional coatings for label-free bioanalysis of blood plasma and serum for medical diagnostics.

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Abbreviations

Ab:

Antibody

ALCAM:

Activated leukocyte cell adhesion molecule

ANACH:

Anachelin

BSA:

Bovine serum albumin

CEA:

Carcinoembryonic antigen

CTAs:

Chain-transfer agents

DOPA:

Dopamine

DSC:

Disuccinimidyl carbonate

EDC:

Ethyl(dimethylaminopropyl) carbodiimide

Fbg:

Fibrinogen

FTIR:

Fourier-transform infrared spectroscopy

hCG:

Human chorionic gonadotropin

HSA:

Human serum albumin

IgG:

Immunoglobulin G

LOD:

Limit of detection

LSPR:

Localized surface plasmon resonance

Lys:

Lysozyme

NHS:

N-Hydroxysuccinimide

OEG:

Oligo(ethylene glycol)

OWLS:

Optical-waveguide light-mode spectroscopy

PBS:

Phosphate-buffered saline

pCBAA:

Carboxybetaine acrylamide

pCBMA:

Carboxybetaine methacrylate

PEG:

Poly(ethylene glycol)

pHEMA:

Poly(2-hydroxyethyl methacrylate)

pHOEGMA:

Poly(oligo(ethylene glycol) methacrylate)

pHPM:

Poly(3-hydroxypropyl methacrylate)

pHPMA:

Poly(N-(2-hydroxypropyl) methacrylamide)

PLL:

Poly(l-lysine)

pMeOEGMA:

Poly(oligo(ethylene glycol) methyl ether methacrylate)

pMPC:

Poly(methacryloyloxyethyl phosphorylcholine

pSBMA:

Poly(sulfobetaine methacrylate)

RAFT:

Reversible addition-fragmentation transfer polymerization

SAMs:

Self-assembled monolayers

SET-LRP:

Electron-transfer living radical polymerization

SI-ATRP:

Surface-initiated atom-transfer radical polymerization

SPR:

Surface plasmon resonance

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Acknowledgements

We thank Cesar Rodriguez-Emmenegger (Institute of Macromolecular Chemistry) and Nicholas Scott Lynn (Institute of Photonics and Electronics) for valuable comments and discussions. This research was supported by Praemium Academiae of the Academy of Sciences of the Czech Republic and the Czech Science Foundation (contract # P205/12/G118).

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Authors and Affiliations

  1. Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague 8, 18251, Czech Republic

    Hana Vaisocherová & Jiří Homola

  2. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky sq.2, Prague 6, 16206, Czech Republic

    Eduard Brynda

Authors
  1. Hana Vaisocherová
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  2. Eduard Brynda
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  3. Jiří Homola
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Correspondence to Jiří Homola.

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Published in the topical collection Direct Optical Detection with guest editors Guenter Gauglitz and Jiri Homola.

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Vaisocherová, H., Brynda, E. & Homola, J. Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications. Anal Bioanal Chem 407, 3927–3953 (2015). https://doi.org/10.1007/s00216-015-8606-5

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  • DOI: https://doi.org/10.1007/s00216-015-8606-5

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