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Ultrasensitive electrochemiluminescent immunosensor based on dual signal amplification strategy of gold nanoparticles-dotted graphene composites and CdTe quantum dots coated silica nanoparticles

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Abstract

A facile and ultrasensitive electrochemiluminescent (ECL) immunosensor for detection of prostate-specific antigen (PSA) was designed by using CdTe quantum dots coated silica nanoparticles (SiO2@QDs) as bionanolabels. To construct such an electrochemiluminescence immunosensor, gold nanoparticles-dotted graphene composites were immobilized on the working electrode, which can increase the surface area to capture a large amount of primary antibodies as well as improve the electronic transmission rate. The as-prepared SiO2@QDs used as bionanolabels, showed good ECL performance and good ability of immobilization for secondary antibodies. The approach provided a good linear response ranging from 0.005 to 10 ng mL−1 with a low detection limit of 0.0032 ng mL−1. Such immunosensor showed good precision, acceptable stability, and reproducibility. Satisfactory results were obtained for determination of PSA in human serum samples. Therefore, the proposed method provides a new promising platform of clinical immunoassay for other biomolecules.

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Acknowledgments

This work was financially supported by Natural Science Research Foundation of China (21277058, 21175058, 21207048), Natural Science Foundation of Shandong Province, China (ZR2012BZ002) and Technology Development Plan of Shandong Province, China (Grant No. 2011GGB01153).

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Authors and Affiliations

  1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China

    Yan Zhang, Weijian Dai, Fang Liu, Long Li, Meng Li, Mei Yan & Jinghua Yu

  2. Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, 250022, China

    Shenguang Ge

Authors
  1. Yan Zhang
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  2. Weijian Dai
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  3. Fang Liu
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  4. Long Li
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  5. Meng Li
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  6. Shenguang Ge
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  7. Mei Yan
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  8. Jinghua Yu
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Corresponding author

Correspondence to Jinghua Yu.

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Zhang, Y., Dai, W., Liu, F. et al. Ultrasensitive electrochemiluminescent immunosensor based on dual signal amplification strategy of gold nanoparticles-dotted graphene composites and CdTe quantum dots coated silica nanoparticles. Anal Bioanal Chem 405, 4921–4929 (2013). https://doi.org/10.1007/s00216-013-6885-2

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  • DOI: https://doi.org/10.1007/s00216-013-6885-2

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