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Influence of secondary preparative parameters and aging effects on PLGA particle size distribution: a sedimentation field flow fractionation investigation

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Abstract

Poly(lactic-co-glycolic acid) particles in the 200–400-nm size range were formulated through nanoprecipitation and solvent evaporation methods. Different concentrations of the polymer and stabilizer (Pluronic® F 68) were tested in order to identify the best conditions for making poly(lactic-co-glycolic acid) particles of suitable size, stable in time, and to be used as carriers for brain-targeting drugs. The particles with the best characteristics for delivery system design were those formulated by nanoprecipitation with an organic/water phase ratio of 2:30, a polymer concentration of 25 mg/mL, and a surfactant concentration of 0.83 mg/mL; their surface charge was reasonably negative (approximately -27 mV) and the average size of the almost monodisperse population was roughly 250 nm. Particle characterization was obtained through ζ-potential measurements, scanning electron microscope observations, and particle size distribution determinations; the latter achieved by both photon-correlation spectroscopy and sedimentation field flow fractionation. Sedimentation field flow fractionation, which is considered more reliable than photon-correlation spectroscopy in describing the possible particle size distribution modifications, was used to investigate the effects of 3 months of storage at 4 °C had on the lyophilized particles.

Particle size ditribution from the SdFFF and the PCS techniques

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Acknowledgements

This work was financially supported by Unife FAR 2010 and by PRIN2009ZSC5K2_004. Thanks are due to M. Hanuskova for her kind technical support with the PCS analysis.

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Authors and Affiliations

  1. Department of Chemistry, University of Ferrara, Via L.Borsari 46, 44121, Ferrara, Italy

    Catia Contado

  2. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy

    Eleonora Vighi & Eliana Leo

  3. Department of Pharmaceutical Sciences, University of Ferrara, Via Fossato di Mortara 17/19, 44121, Ferrara, Italy

    Alessandro Dalpiaz

Authors
  1. Catia Contado
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  2. Eleonora Vighi
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  3. Alessandro Dalpiaz
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  4. Eliana Leo
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Corresponding author

Correspondence to Catia Contado.

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Published in the special issue Analytical Science in Italy with guest editor Aldo Roda.

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Contado, C., Vighi, E., Dalpiaz, A. et al. Influence of secondary preparative parameters and aging effects on PLGA particle size distribution: a sedimentation field flow fractionation investigation. Anal Bioanal Chem 405, 703–711 (2013). https://doi.org/10.1007/s00216-012-6113-5

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  • DOI: https://doi.org/10.1007/s00216-012-6113-5

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