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Using laser ablation/inductively coupled plasma mass spectrometry to bioimage multiple elements in mouse tumors after hyperthermia

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Abstract

In this study, we employed laser ablation/inductively coupled plasma mass spectrometry (LA-ICP-MS) to map the spatial distribution of Gd-doped iron oxide nanoparticles (IONPs) in one tumor slice that had been subjected to magnetic fluid hyperthermia (MFH). The mapping results revealed the high resolution of the elemental analysis, with the distribution of Gd atoms highly correlated with that of the Fe atoms. The spatial distributions of C, P, S, and Zn atoms revealed that the effect of MFH treatment was significantly dependent on the diffusion of the magnetic fluid in the tissue. An observed enrichment of Cu atoms after MFH treatment was probably due to inflammation in the tumor. The abnormal distribution of Ni atoms suggests a probable biochemical reaction in the tumor. Therefore, this LA-ICP-MS mapping technique can provide novel information regarding the spatial distribution of elements in tumors after cancer therapy.

Mapping and ion intensities of a 56Fe and b 158Gd atoms. The red line indicates the path taken during the time-resolved analyses of Fe and Gd atoms

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Acknowledgements

We thank the National Science Council, Taiwan, for funding this project (contract number NSC99-2113-M-007-017), the Instrument Center at National Tsing Hua University for the ICP-MS support, and Professor C. S. Chiang (Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University) for his valuable assistance and insight.

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Authors and Affiliations

  1. Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan

    Yi-Kong Hsieh, Pei-Shin Jiang, Bing-Shen Yang, Hsu-Hsia Peng & Chu-Fang Wang

  2. Department of Environmental Science and Engineering, Tsinghua University, Beijing, 100084, China

    Tian-Ye Sun

  3. Institute of Nuclear Engineering and Science, National Tsing Hua University, Hsinchu, 30013, Taiwan

    Chu-Fang Wang

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  1. Yi-Kong Hsieh
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  2. Pei-Shin Jiang
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Correspondence to Chu-Fang Wang.

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Hsieh, YK., Jiang, PS., Yang, BS. et al. Using laser ablation/inductively coupled plasma mass spectrometry to bioimage multiple elements in mouse tumors after hyperthermia. Anal Bioanal Chem 401, 909–915 (2011). https://doi.org/10.1007/s00216-011-5144-7

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  • DOI: https://doi.org/10.1007/s00216-011-5144-7

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