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Rapid data analysis method for differential mobility cytometry

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Abstract

Differential mobility cytometry (DMC) has recently been established as a powerful method to capture cells and study adhesion processes. DMC uses an oscillation system and cell affinity chromatography to monitor cells as they adhere to a surface. In the past, differential images had to be created individually which limited the throughput of the method. A new method to create differential images is presented. The method involves the subtraction of short movies from each other to create a stack of differential images that can be easily analyzed. In the future, this method will make DMC more accessible and improve throughput.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, 79409, USA

    Randall D. Reif, Kelong Wang & Dimitri Pappas

Authors
  1. Randall D. Reif
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  2. Kelong Wang
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  3. Dimitri Pappas
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Corresponding author

Correspondence to Dimitri Pappas.

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Reif, R.D., Wang, K. & Pappas, D. Rapid data analysis method for differential mobility cytometry. Anal Bioanal Chem 395, 2411–2413 (2009). https://doi.org/10.1007/s00216-009-3154-5

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  • DOI: https://doi.org/10.1007/s00216-009-3154-5

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