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Point-of-care testing of proteins

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Abstract

Point-of-care testing (POCT) is a fast developing area in clinical diagnostics that is considered to be one of the main driving forces for the future in vitro diagnostic market. POCT means decentralized testing at the site of patient care. The most important POCT devices are handheld blood glucose sensors. In some of these sensors, after the application of less than 1 µl whole blood, the results are displayed in less than 10 s. For protein determination, the most commonly used devices are based on lateral flow technology. Although these devices are convenient to use, the results are often only qualitative or semiquantitative. The review will illuminate some of the current methods employed in POCT for proteins and will discuss the outlook for techniques (e.g., electrochemical immunosensors) that could have a great impact on future POCT of proteins.

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Acknowledgement

The author would like to thank Mark Humble (Combimatrix Corp.) for providing the photos in Fig. 9 and the Bundesministerium für Forschung und Technologie (Innoprofile- FKZ 03IP515) for financial support.

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  1. iPOC Research Group, University of Potsdam, Institute of Biochemistry and Biology, Building 25, Karl-Liebknecht-Str. 24-25, 14476, Golm, Germany

    Axel Warsinke

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Correspondence to Axel Warsinke.

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Warsinke, A. Point-of-care testing of proteins. Anal Bioanal Chem 393, 1393–1405 (2009). https://doi.org/10.1007/s00216-008-2572-0

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