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Analytical characterization of chitosan nanoparticles for peptide drug delivery applications

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Abstract

Chitosan-cyclodextrin hybrid nanoparticles (NPs) were obtained by the ionic gelation process in the presence of glutathione (GSH), chosen as a model drug. NPs were characterized by means of transmission electron microscopy and zeta-potential measurements. Furthermore, a detailed X-ray photoelectron spectroscopy study was carried out in both conventional and depth-profile modes. The combination of controlled ion-erosion experiments and a scrupulous curve-fitting approach allowed for the first time the quantitative study of the GSH in-depth distribution in the NPs. NPs were proven to efficiently encapsulate GSH in their inner cores, thus showing promising perspectives as drug carriers.

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References

  1. Rinaudo M (2006) Prog Polym Sci 31:603–632

    Article  CAS  Google Scholar 

  2. Zhang M, Yang H, Rui C (2003) J Bioact Compat Polym 18:391–400

    Article  CAS  Google Scholar 

  3. Koide SS (1998) Nutr Res 18:1091–1101

    Article  CAS  Google Scholar 

  4. Kim DG, Jeong Y, Choi C, Roh SH, Kang SK, Jang MK, Nah JW (2006) Int J Pharm 319:130–138

    Article  CAS  Google Scholar 

  5. Sinha R, Singla AK, Wadhawan S, Kaushik R, Kumria R, Bansal K, Dhawan S (2004) Int J Pharm 274:1–33

    Article  CAS  Google Scholar 

  6. Hejazi R, Amiji M (2003) J Control Release 89:151–165

    Article  CAS  Google Scholar 

  7. Felt O, Buri P, Gurny R (1998) Drug Dev Ind Pharm 24:979–993

    Article  CAS  Google Scholar 

  8. Chandy T, Wilson RF, Rao GHR, Das GS (2002) J Biomater Appl 16:275–291

    Article  CAS  Google Scholar 

  9. Janes KA, Fresneau MP, Marazuela A, Fabra A, Alonso MJ (2001) J Control Release 73:255–267

    Article  CAS  Google Scholar 

  10. Denkbas EB, Ottenbrite RM (2006) J Bioact Compat Polym 21:351–368

    Article  CAS  Google Scholar 

  11. Calvo P, Remuñan-Lopez C, Vila JL, Alonso MJ (1997) J Appl Polym Sci 63:125–132

    Article  CAS  Google Scholar 

  12. Andersson ME (1998) Chem Biol Interact 111–112:1–14

    Article  Google Scholar 

  13. Konorev E, Joseph J, Kalyanaraman B (1997) Biochem J 323:477–481

    Google Scholar 

  14. Janes KA, Calvo P, Alonso MJ (2001) Adv Drug Deliv Rev 47:83–97

    Article  CAS  Google Scholar 

  15. Krauland AH, Alonso MJ (2007) Int J Pharm 340:134–142

    Article  CAS  Google Scholar 

  16. Brindley A, Davis SS, Davies MC, Watts JF (1995) J Colloid Interface Sci 171:150–161

    Article  CAS  Google Scholar 

  17. John CM, Odom RW, Salvati L, Annapragada A, Lu MYF (1995) Anal Chem 67:3871–3878

    Article  CAS  Google Scholar 

  18. Carli F, Colombo I, Lovrecich M, Rubessa F, Torricelli C (1986) Polym Sci Technol 34(2):397–407

    CAS  Google Scholar 

  19. Garbassi F, Carli F (1986) Surf Interface Anal 8:229–233

    Article  CAS  Google Scholar 

  20. Chesko J, Kazzaz J, Ugozzoli M, Singh M, O’Hagan DT, Madden C, Perkins M, Patel N (2008) J Pharm Sci 97:1443–1453

    Article  CAS  Google Scholar 

  21. Morales ME, Ruiz MA, Oliva I, Oliva M, Gallardo V (2007) Int J Pharm 333:162–166

    Article  CAS  Google Scholar 

  22. Zhang Z, Lee SH, Feng SS (2007) Biomaterials 28:1889–1899

    Article  Google Scholar 

  23. Layre A, Couvreur P, Chacun H, Richard J, Passirani C, Requier D, Benoit JP, Gref R (2006) J Control Release 111:271–280

    Article  CAS  Google Scholar 

  24. Zhang Z, Feng SS (2006) Biomacromolecules 7:1139–1146

    Article  CAS  Google Scholar 

  25. Mu L, Seow PH, Ang SN, Feng SS (2004) Colloid Polym Sci 283:58–65

    Article  CAS  Google Scholar 

  26. Passerini N, Perissutti B, Albertini B, Voinovich D, Moneghini M, Rodriguez L (2003) J Control Release 88:263–275

    Article  CAS  Google Scholar 

  27. Feng SS, Li M, Chen BH, Pack D (2002) Mater Sci Eng C Biomimetic Supramol Syst C20:85–92

    CAS  Google Scholar 

  28. Appawu JA, Yu J, Gardella JA, Wei G, Bobek LA (2006) In: Abstracts of papers, 232nd ACS national meeting, San Francisco, United States, Sept. 10–14, 2006, PMSE-259

  29. Grenha A, Seijo B, Serra C, Remunan-Lopez C (2007) Biomacromolecules 8:2072–2079

    Article  CAS  Google Scholar 

  30. Grenha A, Seijo B, Serra C, Remunan-Lopez C (2008) J Nanosci Nanotechnol 8:358–365

    CAS  Google Scholar 

  31. Loretz B, Thaler M, Bernkop-Schnuerch A (2007) Bioconjug Chem 18:1028–1035

    Article  CAS  Google Scholar 

  32. El-Gibaly I, Meki AMA, Abdel-Ghaffar SK (2003) Int J Pharm 260:5–22

    Article  CAS  Google Scholar 

  33. Krauland AH Leitner VM, Grabovac V, Bernkop-Schnurch A (2006) J Pharm Sci 95:2463–2472

    Article  Google Scholar 

  34. Hoyer H, Schlocker W, Krum Ka, Bernkop-Schnuerch A (2008) Eur J Pharm Biopharm 69:476–485

    Article  CAS  Google Scholar 

  35. Di Colo G, Zambito Y, Zaino C (2008) J Pharm Sci 97:1652–1680

    Article  Google Scholar 

  36. Krauland AH, Guggi D, Bernkop-Schnuerch A (2006) Int J Pharm 307:270–277

    Article  CAS  Google Scholar 

  37. Timur S, Odaci D, Dincer A, Zihnioglu F, Telefoncu A (2008) Talanta 74:1492–1497

    Article  CAS  Google Scholar 

  38. Trapani A, Garcia-Fuentes M, Alonso MJ (2008) Nanotechnology 19:185101/1–185101/10

    Article  CAS  Google Scholar 

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Acknowledgement

Thanks are due to G. Trapani (Pharmaco-Chemistry Department, University of Bari) for helpful discussions.

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Authors and Affiliations

  1. Department of Chemistry, University of Bari, Via Orabona 4, 70126, Bari, Italy

    E. Ieva, N. Cioffi, N. Ditaranto, A. Monopoli & L. Sabbatini

  2. Pharmaco-Chemistry Department, University of Bari, Via Orabona 4, 70126, Bari, Italy

    A. Trapani

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  1. E. Ieva
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  2. A. Trapani
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  3. N. Cioffi
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  4. N. Ditaranto
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Correspondence to N. Cioffi.

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Ieva, E., Trapani, A., Cioffi, N. et al. Analytical characterization of chitosan nanoparticles for peptide drug delivery applications. Anal Bioanal Chem 393, 207–215 (2009). https://doi.org/10.1007/s00216-008-2463-4

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  • DOI: https://doi.org/10.1007/s00216-008-2463-4

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