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Studies on interactions of programmed cell death 5 (PDCD5) and its related peptides with heparin by capillary zone electrophoresis

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Abstract

Capillary zone electrophoresis (CZE) was used to investigate interactions between heparin and programmed cell death 5 (PDCD5), and between heparin and PDCD5-related peptides. Samples containing PDCD5, PDCD5-related peptides, and heparin at various ratios were incubated at room temperature and then separated by CZE with tris-acetate buffer at pH 7.2. Both qualitative and quantitative characterizations of the binding of PDCD5 and PDCD5-related peptides to heparin were determined. The changes in the signals of PDCD5 and PDCD5-related peptides were monitored by comparing the electropherograms of the mixtures containing PDCD5 and heparin and PDCD5-related peptides and heparin with that of PDCD5 or PDCD5-related peptides only. The binding constant of the interaction between PDCD5 and heparin was calculated as 4.17 × 104 M−1 by Scatchard analysis. Our investigations show that it is possible to characterize the interaction between PDCD5 and heparin quantitatively and the interaction between PDCD5-related peptides and heparin qualitatively using CZE.

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Acknowledgements

This work was financially supported by the National High-Tech Research and Development Program of China (863 Program) (2004AA2Z3783 and 2002BA711A01) and a grant from the State Key Program of Basic Research (G2000056909).

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, Beijing, 100083, China

    Xiaomei Ling & Yi Liu

  2. Center for Human Disease Genomics, Peking University, Beijing, 100083, China

    Hui Fan, Yingcheng Zhong, Dan Li & Ying Wang

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  1. Xiaomei Ling
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  2. Yi Liu
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  3. Hui Fan
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  4. Yingcheng Zhong
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  5. Dan Li
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  6. Ying Wang
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Correspondence to Xiaomei Ling or Ying Wang.

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Ling, X., Liu, Y., Fan, H. et al. Studies on interactions of programmed cell death 5 (PDCD5) and its related peptides with heparin by capillary zone electrophoresis. Anal Bioanal Chem 387, 909–916 (2007). https://doi.org/10.1007/s00216-006-0983-3

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  • DOI: https://doi.org/10.1007/s00216-006-0983-3

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