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Rapid and simultaneous determination of nonsteroidal anti-inflammatory drugs in human plasma by LC–MS with solid-phase extraction

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Abstract

A rapid, selective and sensitive analytical method for the simultaneous determination of 16 nonsteroidal anti-inflammatory drugs (NSAIDs) in human plasma was carried out using Oasis HLB solid-phase extraction (SPE), followed by reversed-phase high-performance liquid chromatography and quadrupole mass spectrometry with electrospray ionization operated in the negative ion mode. The recoveries of NSAIDs from human plasma by SPE were greater than 76.7%. The use of a short column packed with small particles enabled rapid and simultaneous determination within 7 min. The detection limits for the NSAIDs were 0.01–0.9 μg/ml using the selected ion monitoring mode.

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References

  1. Shinozuka T, Terada M, Ogamo A, Nakajima R, Takei S, Murai T, Wakasugi C, Yanagida J (1996) Jpn J Forensic Toxicol 14:246–252

    Google Scholar 

  2. Battista HJ, Wehinger G, Henn R (1985) J Chromatogr 345:77–89

    PubMed  Google Scholar 

  3. Owen SG, Roberts MS, Friesen WT (1987) J Chromatogr 416:293–302

    PubMed  Google Scholar 

  4. Taylor MR, Westwood SA (1995) J Chromatogr A 697:389–396

    Article  Google Scholar 

  5. Hirai T, Matsumoto S, Kishi I (1997) J Chromatogr B 692:375–388

    Google Scholar 

  6. Arcelloni C, Lanzi R, Pedercini S, Molteni G, Fermo I, Pontiroli A, Paroni R (2001) J Chromatogr B 763:195–200

    Google Scholar 

  7. Martín MJ, Pablos F, González AG (1999) Talanta 49:453–459

    Article  Google Scholar 

  8. Kuhlmann O, Stoldt G, Struck H, Krauss G (1998) J Pharm Biomed Anal 17:1351–1356

    Article  PubMed  Google Scholar 

  9. Gonzátlez G, Ventura R, Smith AK, Torre R, Segura J (1996) J Chromatogr A 719:251–264

    Article  PubMed  Google Scholar 

  10. Rodríguez I, Carpinteiro J, Quintana JB, Carro AM, Lorenzo RA, Cela R (2004) J Chromatogr A 1024:1–8

    Google Scholar 

  11. Jin W, Zhang J (2000) J Chromatogr A 868:101–107

    Article  PubMed  Google Scholar 

  12. Shinozuka T, Takei S, Kuroda N, Ogamo A, Terada M, Wakasugi C, Yanagida J (1996) Jpn J Forensic Toxicol 14:43–51

    Google Scholar 

  13. Ahrer W, Scherwenk E, Buchberger W (2001) J Chromatogr A 910:69–78

    Article  PubMed  Google Scholar 

  14. Abdel-Hamid ME, Novotny L, Hamza H (2001) J Pharm Biomed Anal 24:587–594

    Article  PubMed  Google Scholar 

  15. Mayer BX, Namiranian K, Dehghanyar P, Stroh R, Mascher H, Müller M (2003) J Pharm Biomed Anal 33:745–754

    Article  PubMed  Google Scholar 

  16. Bonato PS, Del Lama MPFM, de Carvalho R (2003) J Chromatogr B 796:413–420

    Article  Google Scholar 

  17. Hoke SH II, Pinkston JD, Bailey RE, Tanguay SL, Eichhold TH (2000) Anal Chem 72:4235–4241

    Article  PubMed  Google Scholar 

  18. Petroviæ M, Hernando MD, Díaz-Cruz MS, Barceló D (2005) J Chromatogr A 1067:1–14

    Article  PubMed  Google Scholar 

  19. Moffat AC, Osselton MD, Widdop B (2004) Clarke’s analysis of drugs and poisons in pharmaceuticals, body fluids and postmortem material, 3rd edn. Pharmaceutical Press, London

Download references

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Koichi Suenami, Lee Wah Lim & Toyohide Takeuchi

  2. Criminal Investigation Laboratory, Gifu Pref. Police H.Q., 2-1-1 Yabutaminami, Gifu, 500–8501, Japan

    Koichi Suenami, Yasuhide Sasajima, Kiyohito Sato, Yuji Takekoshi & Susumu Kanno

Authors
  1. Koichi Suenami
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  2. Lee Wah Lim
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  3. Toyohide Takeuchi
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  4. Yasuhide Sasajima
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  5. Kiyohito Sato
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  6. Yuji Takekoshi
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  7. Susumu Kanno
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Correspondence to Koichi Suenami.

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Suenami, K., Lim, L.W., Takeuchi, T. et al. Rapid and simultaneous determination of nonsteroidal anti-inflammatory drugs in human plasma by LC–MS with solid-phase extraction. Anal Bioanal Chem 384, 1501–1505 (2006). https://doi.org/10.1007/s00216-006-0352-2

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  • DOI: https://doi.org/10.1007/s00216-006-0352-2

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