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Structural levansucrase gene (lsdA) constitutes a functional locus conserved in the species Gluconacetobacter diazotrophicus

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Abstract.

Levansucrase (EC 2.4.1.10) was identified as a constitutive exoenzyme in 14 Gluconacetobacter diazotrophicus strains recovered from different host plants in diverse geographical regions. The enzyme, consisting of a single 60-kDa polypeptide, hydrolysed sucrose to synthesise oligofructans and levan. Sugar-cane-associated strains of the most abundant genotype (electrophoretic type 1) showed maximal values of levansucrase production. These values were three-fold higher than those of the isolates recovered from coffee plants. Restriction fragment length polymorphism analysis revealed a high degree of conservation of the levansucrase locus (lsdA) among the 14 strains under study, which represented 11 different G. diazotrophicus genotypes. Targeted disruption of the lsdA gene in four representative strains abolished their ability to grow on sucrose, indicating that the endophytic species G. diazotrophicus utilises plant sucrose via levansucrase.

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Authors and Affiliations

  1. Centro de Ingeniería Genética y Biotecnología, División de Plantas, Laboratorio de Interacciones Planta-Microorganismos. Apartado 6162, Habana 10600, Cuba, , , , ,

    Lázaro Hernández, Mailin Sotolongo, Yamilka Rosabal, Carmen Menéndez, Ricardo Ramírez & Juan Arrieta

  2. Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, México, , , , ,

    Jesús Caballero-Mellado

Authors
  1. Lázaro Hernández
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  2. Mailin Sotolongo
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  3. Yamilka Rosabal
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  4. Carmen Menéndez
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  5. Ricardo Ramírez
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  6. Jesús Caballero-Mellado
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  7. Juan Arrieta
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Hernández, L., Sotolongo, M., Rosabal, Y. et al. Structural levansucrase gene (lsdA) constitutes a functional locus conserved in the species Gluconacetobacter diazotrophicus. Arch Microbiol 174, 120–124 (2000). https://doi.org/10.1007/s002030000173

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  • DOI: https://doi.org/10.1007/s002030000173

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