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Cell surface structure enhancing uptake of polyvinyl alcohol (PVA) is induced by PVA in the PVA-utilizing Sphingopyxis sp. strain 113P3

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Abstract

Polyvinyl alcohol (PVA)-utilizing Sphingopyxis sp. 113P3 (reidentified from Sphingomonas sp. 113P3) removed almost 0.5% PVA from culture supernatants in 4 days. Faster degradation of 0.5% PVA was performed by the periplasmic fraction. The average molecular size of PVA in the culture supernatant or cell-bound PVA was gradually shifted higher, suggesting that lower molecular size molecules are degraded faster. Depolymerized products were found in neither the culture supernatant nor the cell-bound fraction; however they were recovered from the periplasmic fraction. As extracellular or cell-associated PVA oxidase activity was almost undetectable in strain 113P3, degradation of PVA must be performed by periplasmic PVA dehydrogenase after uptake into the periplasm. Following the consumption of PVA, a dent appeared on the cell surface on day 2 and increased in size and depth for 4 days and was maintained for 8 days. Ultrastructural change on the cell surface was only observed in PVA medium, but not in nutrient broth (NB), suggesting that the change is induced by PVA. Fluorescein-4-isothiocyanate-labeled PVA was bound more to cells grown in PVA than to cells grown in NB. No binding was found with PVA-grown cells treated with formaldehyde. Thus, a dent on the cell surface seems to be related to the uptake of PVA.

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Authors and Affiliations

  1. Laboratory of Applied Microbiology, Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama, 710-0046, Japan

    Xiaoping Hu, Yumi Shimomura, Kazuhide Kimbara & Fusako Kawai

  2. College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, 712100, People’s Republic of China

    Xiaoping Hu

  3. Faculty of Nutrition, Kobe Gakuin University, Kobe, Hyogo, 651-2180, Japan

    Rie Mamoto

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  1. Xiaoping Hu
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  2. Rie Mamoto
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  3. Yumi Shimomura
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  4. Kazuhide Kimbara
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  5. Fusako Kawai
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Correspondence to Fusako Kawai.

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Communicated by Jorge Membrillo-Hernández.

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Hu, X., Mamoto, R., Shimomura, Y. et al. Cell surface structure enhancing uptake of polyvinyl alcohol (PVA) is induced by PVA in the PVA-utilizing Sphingopyxis sp. strain 113P3. Arch Microbiol 188, 235–241 (2007). https://doi.org/10.1007/s00203-007-0239-4

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  • DOI: https://doi.org/10.1007/s00203-007-0239-4

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