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Transcriptional and Biochemical Alterations in Zebrafish Eleuthero-Embryos (Danio rerio) After Exposure to Synthetic Progestogen Dydrogesterone

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Abstract

Little information has so far been known on the effects of synthetic progestogen dydrogesterone (DDG) in organisms like fish. This study aimed to investigate the effects of DDG on the transcriptional and biochemical alterations in zebrafish eleuthero-embryos. Zebrafish eleuthero-embryos were analyzed for the transcriptional alterations by real-time quantitative PCR (RT-qPCR) and biochemical changes by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FITR) after 144 h exposure to DDG. The results of qPCR analysis showed that DDG exposure significantly suppressed the transcriptions of target genes involved in hypothalamic–pituitary–thyroid (HPT) axis, while it induced the expression of target genes mRNA belonging to hypothalamic–pituitary–gonad (HPG) axis. In addition, ATR-FTIR spectroscopy analysis showed that the biochemical alterations of protein, nucleic acid and lipid were observed following DDG treatment. The finding from this study suggests that DDG exposure could have potential multiple effects in fish.

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Acknowledgements

The authors would like to acknowledge the financial support from the National Natural Science Foundation of China (41273119, and U1401235), National Water Pollution Control Program (2014ZX07206-005) and GIG CAS (IS-2348).

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Authors and Affiliations

  1. State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 510640, Guangzhou, China

    Wen-Jun Shi, Guang-Guo Ying, Guo-Yong Huang, Yan-Qiu Liang, Li-Xin Hu, Jian-Liang Zhao & Jin-Na Zhang

  2. School of Chemistry and Environment, Guangdong Ocean University, 524088, Zhanjiang, China

    Yan-Qiu Liang

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  1. Wen-Jun Shi
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  2. Guang-Guo Ying
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  3. Guo-Yong Huang
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  4. Yan-Qiu Liang
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  5. Li-Xin Hu
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  6. Jian-Liang Zhao
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  7. Jin-Na Zhang
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Correspondence to Guang-Guo Ying.

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Shi, WJ., Ying, GG., Huang, GY. et al. Transcriptional and Biochemical Alterations in Zebrafish Eleuthero-Embryos (Danio rerio) After Exposure to Synthetic Progestogen Dydrogesterone. Bull Environ Contam Toxicol 99, 39–45 (2017). https://doi.org/10.1007/s00128-017-2046-1

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  • DOI: https://doi.org/10.1007/s00128-017-2046-1

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