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Evaluation of Biochemical, Genetic and Hematological Biomarkers in a Commercial Catfish Rhamdia quelen Exposed to Diclofenac

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Abstract

Juveniles Rhamdia quelen fish species were exposed to diclofenac for 96 h at concentrations of 0.2, 2, and 20 μg/L. Biochemical, genetic, and hematological biomarkers were assessed in the liver, kidney, and blood in order to evaluate the toxic effects. No oxidative stress was observed in liver. In kidney the superoxide dismutase activity increased in all concentrations, suggesting an alteration in the hydrogen peroxide production, but DNA damage and lipid peroxidation were not detected. Diclofenac exposure increased the red blood cells number at concentrations of 0.2 and 2 μg/L, and monocytes and neutrophils at 2 and 20 μg/L, respectively. These results suggest that acute exposure to diclofenac, even at low concentrations, caused hematologic and renal enzymatic alterations in R. quelen.

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Acknowledgments

This work was supported in part by CNPq (Brazilian Agency for Science and Technology) and CAPES (National Council for the Improvement of Higher Education).

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Authors and Affiliations

  1. Ecology and Conservation Program of Post-Graduation, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil

    Ariane Ghelfi

  2. Department of Pharmacology, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil

    João Luiz Coelho Ribas, Izonete Cristina Guiloski, Franciele Lima Bettim & Helena Cristina Silva de Assis

  3. Department of Genetics, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil

    Laércio Dante Stein Piancini & Marta Margarete Cestari

  4. Department of Biochemistry, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil

    Aramis José Pereira & Guilherme Lanzi Sassaki

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  1. Ariane Ghelfi
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  2. João Luiz Coelho Ribas
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  9. Helena Cristina Silva de Assis
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Correspondence to Helena Cristina Silva de Assis.

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Ghelfi, A., Ribas, J.L.C., Guiloski, I.C. et al. Evaluation of Biochemical, Genetic and Hematological Biomarkers in a Commercial Catfish Rhamdia quelen Exposed to Diclofenac. Bull Environ Contam Toxicol 96, 49–54 (2016). https://doi.org/10.1007/s00128-015-1693-3

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  • DOI: https://doi.org/10.1007/s00128-015-1693-3

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