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Utility of Delayed Fluorescence as Endpoint for Rapid Estimation of Effect Concentration on the Green Alga Pseudokirchneriella subcapitata

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Abstract

Algal growth inhibition tests for environmental risk assessment require improved efficiency to evaluate large numbers of chemicals. As an endpoint for rapid estimation of the effect concentration of test chemicals, we propose the delayed fluorescence (DF) measurement from an alga 24 h after exposure. Eight chemicals (bifenox, bromoxynil, bensulfuronmethyl, diuron, diflufenican, thiobencarb, m-chlorophenylhydrazone and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone) were tested. The EC50 values from the 24 h DF measurement were similar to those from the conventional 72 h growth test for seven tested chemicals excepting thiobencarb. We conclude that 24 h DF measurement is a possible endpoint for rapid estimation of the EC50 values obtained in the 72 h growth test for those seven chemicals.

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Acknowledgments

We are deeply grateful to Ayame Maihara and Sachiko Tanaka (JCL Bioassay Corporation, Japan) for their valuable work on the growth inhibition tests. This research was supported in part by the Environmental Technology Development Fund of the Ministry of the Environment of Japan.

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Authors and Affiliations

  1. Central Research Laboratory, Hamamatsu Photonics K. K., 5000 Hirakuchi, Hamakita-Ku, Hamamatsu, Shizuoka, 434-8601, Japan

    Masakazu Katsumata, Kimiko Kazumura & Ayano Takeuchi

  2. Electron Tube Center, Hamamatsu Photonics K. K., 314-5 Shimokanzo, Iwata, Shizuoka, 438-0193, Japan

    Takashi Koike

  3. Research Center for Environmental Risk, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan

    Yoshio Sugaya

Authors
  1. Masakazu Katsumata
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  2. Takashi Koike
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  3. Kimiko Kazumura
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  4. Ayano Takeuchi
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  5. Yoshio Sugaya
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Correspondence to Masakazu Katsumata.

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Katsumata, M., Koike, T., Kazumura, K. et al. Utility of Delayed Fluorescence as Endpoint for Rapid Estimation of Effect Concentration on the Green Alga Pseudokirchneriella subcapitata . Bull Environ Contam Toxicol 83, 484–487 (2009). https://doi.org/10.1007/s00128-009-9826-1

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  • DOI: https://doi.org/10.1007/s00128-009-9826-1

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