Abstract
Two segregating populations for citrus tristeza virus (CTV) resistance derived from Poncirus trifoliata var ‘Flying Dragon’ by self-pollination and pollination to Citrus medica L. var ethrog ‘Arizona’ were inoculated with a common CTV isolate. The presence of virus was checked by the Double Antibody Sandwich Enzyme-Linked Assay and Direct Tissue Blot Inmunoassay at 3, 6, and 12 months after inoculation. Seven RAPDs were found linked to the CTV resistance gene by bulked segregant analysis. The closest linked RAPDs were cloned to obtain linked codominant RFLPs and to increase the precision of the genetic distance estimation. The CTV resistance gene seems to be located between cW18 and cK16. Differences in genetic distances among progenies are large and can be explained by genome-wide reduction in the recombination of progeny derived from male versus female gametes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 5 June 1996 / Accepted: 26 July 1996
Rights and permissions
About this article
Cite this article
Mestre, P., Asíns, M., Pina, J. et al. Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L.) Raf.. Theor Appl Genet 94, 458–464 (1997). https://doi.org/10.1007/s001220050437
Issue Date:
DOI: https://doi.org/10.1007/s001220050437