Abstract
RFLP-based genetic map of rye, developed previously using a cross of lines DS2×RXL10 (F2 generation), was extended with 69 RAPD and 12 isozyme markers. The actual map contains 282 markers dispersed on all seven chromosomes and spans a distance of 1,140 cM. The efficiency of mapping RAPD markers was close to ten loci per 100-screened arbitrary primers. A strong selection of polymorphic, intensive and reproducible fragments was necessary to reveal individual marker loci that could be assigned to rye chromosomes. Newly mapped markers cover a substantial part of the rye genome and constitute a valuable tool suitable for map saturation, marker-aided selection and phenetic studies. A specific nomenclature for the RAPD loci mapped on individual rye chromosomes, which could be helpful in managing of accumulating data, is proposed.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 8 May 2000 / Accepted: 17 October 2000
Rights and permissions
About this article
Cite this article
Masojc´, P., Mys´ków, B. & Milczarski, P. Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers. Theor Appl Genet 102, 1273–1279 (2001). https://doi.org/10.1007/s001220000512
Issue Date:
DOI: https://doi.org/10.1007/s001220000512