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Analysis of the chromosome 2(2H) region of barley associated with the correlated traits Fusarium head blight resistance and heading date

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Abstract

Fusarium head blight (FHB) is a major disease of barley (Hordeum vulgare L.) that results in reduced grain yield and quality through the accumulation of the mycotoxin deoxynivalenol (DON). Coincident QTL for FHB severity, DON concentration, and heading date (HD) map to a region of chromosome 2(2H) designated Qrgz-2H-8. It is unclear whether disease resistance at this locus is due to a pleiotropic effect of late HD by delaying the host exposure to the pathogen or a tightly linked resistance gene. The objectives of this study were to develop a set of near isogenic lines (NILs) for the Qrgz-2H-8 region and to genetically dissect the QTL region containing the coincident traits. Two NIL populations were developed consisting of F2- and F4-derived recombinants from a cross between a BC5 line carrying the donor parent (Chevron) alleles in the Qrgz-2H-8 region and the recurrent parent M69. Analysis of field and marker data from these NILs revealed that the Chevron alleles conditioning FHB resistance, late HD, and low DON concentration were successfully introgressed into the BC5 parent line and were segregating among NILs. QTL analysis of the F4-derived population showed that the HD QTL is adjacent to the FHB QTL. Furthermore, a single NIL was identified that was similar to the resistant BC5 parent for FHB severity and the early flowering parent M69 for HD. These results indicate that the relationship between FHB and HD at the Qrgz-2H-8 region is likely due to tight linkage rather than pleiotropy.

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Acknowledgments

We thank Ed Schiefelbein, Guillermo Velasquez, Charlie Gustus, Ruth Dill-Macky, Amar Elkkad, Karen Wennberg, Shane Heinen, Charla Hollingsworth, and Galen Thompson for technical assistance. We also thank Dr. Bingxin Zhang for establishing the FHB nursery at Zhejiang University in Hangzhou, China. This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No.59-0790-4-120. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. Partial support was also received from the U.S. Barley Genome Project. Additionally, we thank the University of Minnesota Super Computing Center for providing access to the software JoinMap.

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Authors and Affiliations

  1. Department of Agronomy and Plant Genetics, University of Minnesota, Rm 411 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN, 55108, USA

    L. M. Nduulu, A. Mesfin, G. J. Muehlbauer & K. P. Smith

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  1. L. M. Nduulu
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  2. A. Mesfin
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  3. G. J. Muehlbauer
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  4. K. P. Smith
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Correspondence to K. P. Smith.

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Communicated by F. Ordon.

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Nduulu, L.M., Mesfin, A., Muehlbauer, G.J. et al. Analysis of the chromosome 2(2H) region of barley associated with the correlated traits Fusarium head blight resistance and heading date. Theor Appl Genet 115, 561–570 (2007). https://doi.org/10.1007/s00122-007-0590-5

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