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MK17, a specific marker closely linked to the gynoecium suppression region on the Y chromosome in Silene latifolia

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Abstract

The aim of this work was to isolate new DNA markers linked to the Silene latifolia Y chromosome. To do this we created a chromosome-specific plasmid library after DOP-PCR amplification of laser-microdissected Y-chromosomes. The library screening led to the isolation of several clones yielding mostly to exclusive male specific hybridization signals. Subsequent PCR confirmed the Y-unique linkage for one of the sequences. This DNA sequence called MK17 has no homology to any known DNA sequence and it is not expressed. Based on PCR and Southern analyses, MK17 is present only in dioecious species of the Elisanthe section of the genus Silene (S. latifolia, S. dioica, and S. diclinis) and it is absent in related gynodioecious and hermaphroditic species. The mapping analysis using a panel of deletion mutants showed that MK17 is closely linked to the region controlling suppression of gynoecium development. Hence MK17 represents a valuable marker to isolate genes controlling the gynoecium development suppression on the Y chromosome of S. latifolia.

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References

  • Atanassov I, Delichere C, Filatov DA, Charlesworth D, Negrutiu I, Moneger F (2001) Analysis and evolution of two functional Y-linked loci in a plant sex chromosome system. Mol Biol Evol 18:2162–2168

    PubMed  CAS  Google Scholar 

  • Barbacar N, Hinnisdaels S, Farbos I, Moneger F, Lardon A, Delichere C, Mouras A, Negrutiu I (1997) Isolation of early genes expressed in reproductive organs of the dioecious white campion (Silene latifolia) by subtraction cloning using an asexual mutant. Plant J 12:805–817

    Article  PubMed  CAS  Google Scholar 

  • Buzek J, Koutnikova H, Houben A, Riha K, Janousek B, Siroky J, Grant S, Vyskot B (1997) Isolation and characterization of X chromosome-derived DNA sequences from a dioecious plant Melandrium album. Chromosome Res 5:57–65

    Article  PubMed  CAS  Google Scholar 

  • Charlesworth B, Charlesworth D (2000) The degeneration of Y chromosomes. Phil Trans R Soc Lond B Biol Sci 355:1563–1572

    Article  CAS  Google Scholar 

  • Charlesworth D, Charlesworth B, Marais G. (2005) Steps in the evolution of heteromorphic sex chromosomes. Heredity 95:118–128

    Article  PubMed  CAS  Google Scholar 

  • Delichére C, Veuskens J, Hernould M, Barbacar N, Mouras A, Negrutiu I, Moneger F (1999) SlY1, the first active gene cloned from a plant Y chromosome, encodes a WD-repeat protein. EMBO J 18:4169–4179

    Article  PubMed  Google Scholar 

  • Donnison IS, Siroky J, Vyskot B, Saedler H, Grant SR (1996) Isolation of Y chromosome-specific sequences from Silene latifolia and mapping of male sex-determining genes using representational difference analysis. Genetics 144:1893–1901

    PubMed  CAS  Google Scholar 

  • Farbos I, Veuskens J, Vyskot B, Oliveira M, Hinnisdaels S, Aghmir A, Mouras A, Negrutiu I (1999) Sexual dimorphism in white campion: deletion on the Y chromosome results in a floral asexual phenotype. Genetics 151:1187–1196

    PubMed  CAS  Google Scholar 

  • Filatov DA (2005) Substitution rates in a new Silene latifolia sex-linked gene, SlssX/Y. Mol Biol Evol 22:402–408

    Article  PubMed  CAS  Google Scholar 

  • Filatov DA, Charlesworth D (2002) Substitution rates in the X- and Y-linked genes of the plants, Silene latifolia and S. dioica. Mol Biol Evol 19:898–907

    PubMed  CAS  Google Scholar 

  • Filatov DA, Moneger F, Negrutiu I, Charlesworth D (2000) Low variability in a Y-linked plant gene and its implications for Y-chromosome evolution. Nature 404:388–390

    Article  PubMed  CAS  Google Scholar 

  • Guttman DS, Charlesworth D (1998) An X-linked gene with a degenerate Y-linked homologue in a dioecious plant. Nature 393:263–266

    Article  PubMed  CAS  Google Scholar 

  • Gvozdev VA, Kogan GL, Usakin LA (2005) The Y chromosome as a target for acquired and amplified genetic material in evolution. Bioessays 27:1256–1262

    Article  PubMed  CAS  Google Scholar 

  • Hladilova R, Siroky J, Vyskot B (1998) A cytospine technique for spreading plant metaphases suitable for immunofluorescence studies. Biotechnic Histochem 73:150–156

    Article  CAS  Google Scholar 

  • Hobza R, Lengerova M, Cernohorska H, Rubes J, Vyskot B (2004) FAST-FISH with laser beam microdissected DOP-PCR probe distinguishes the sex chromosomes of Silene latifolia. Chromosome Res 12:245–250

    Article  PubMed  CAS  Google Scholar 

  • Kejnovsky E, Vrana J, Matsunaga S, Soucek P, Siroky J, Dolezel J, Vyskot B (2001) Localization of male-specifically expressed MROS genes of Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes. Genetics 158:1269-1277

    PubMed  CAS  Google Scholar 

  • Kubickova S, Cernohorska H, Musilova P, Rubes J (2001) The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals. Chromosome Res 10:571-577

    Article  Google Scholar 

  • Lardon A, Georgiev S, Aghmir A, Le Merrer G, Negrutiu I (1999) Sexual dimorphism in white campion: complex control of carpel number is revealed by Y chromosome deletions. Genetics 3:1173–1185

    Google Scholar 

  • Lebel-Hardenack S, Hauser E, Law TF, Schmid J, Grant SR (2002) Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism. Genetics 160:717–725

    PubMed  CAS  Google Scholar 

  • Lengerova M, Kejnovsky E, Hobza R, Macas J, Grant SR, Vyskot B (2004) Multicolor FISH mapping of the dioecious model plant, Silene latifolia. Theor Appl Genet 108:1193–1199

    Article  PubMed  CAS  Google Scholar 

  • Marin I, Baker BS (1998) The evolutionary dynamics of sex determination. Science 281:1990–1994

    Article  PubMed  CAS  Google Scholar 

  • Matsunaga S, Kawano S, Takano H, Uchida H, Sakai A, Kuroiwa T (1996) Isolation and developmental expression of male reproductive organ-specific genes in a dioecious campion, Melandrium album (Silene latifolia). Plant J 10:679–689

    Article  PubMed  CAS  Google Scholar 

  • Matsunaga S, Kawano S, Michimoto T, Higashiyama T, Nakao S, Sakai A, Kuroiwa T (1999) Semi-automatic laser beam microdissection of the Y chromosome and analysis of Y chromosome DNA in a dioecious plant, Silene latifolia. Plant Cell Physiol 40:60–68

    PubMed  CAS  Google Scholar 

  • Matsunaga S, Isono E, Kejnovsky E, Vyskot B, Dolezel J, Kawano S, Charlesworth D (2003) Duplicative transfer of a MADS box gene to a plant Y chromosome. Mol Biol Evol 20:1062–1069

    Article  PubMed  CAS  Google Scholar 

  • Moore RC, Kozyreva O, Lebel-Hardenack S, Siroky J, Hobza R, Vyskot B, Grant SR (2003) Genetic and functional analysis of DD44, a sex-linked gene from dioecious plant Silene latifolia, provides clues to early events in sex chromosome evolution. Genetics 163:321–334

    PubMed  CAS  Google Scholar 

  • Mulcahy DL , Weeden NF, Kesseli RV, Carroll SB (1992) DNA probes for the Y-chromosome of Silene latifolia, a dioecious angiosperm. Sex Plant Reprod 3:1–3

    Google Scholar 

  • Nakao S, Matsunaga S, Sakai A, Kuroiwa T, Kawano S (2002) RAPD isolation of a Y chromosome specific ORF in a dioecious plant, Silene latifolia. Genome 45:413–420

    Article  PubMed  CAS  Google Scholar 

  • Negrutiu I, Vyskot B, Barbacar N, Georgiev S, Moneger F (2001) Dioecious plants. A key to the early events of sex chromosome evolution. Plant Physiol 127:1418–1424

    Article  PubMed  CAS  Google Scholar 

  • Nicolas M, Marais G, Hykelova V, Janousek B, Laporte V, Vyskot B, Mouchiroud D, Negrutiu I, Charlesworth D, Moneger F (2005) A gradual and ongoing process of recombination restriction in the evolutionary history of the sex chromosomes in dioecius plants. Publ Libr Sci Biol 3:47–56

    CAS  Google Scholar 

  • Pritham EJ, Zhang YH, Feschotte C, Kesseli RV (2003) An Ac-like transposable element family with transcriptionally active Y-linked copies in the white campion, Silene latifolia. Genetics 165:799–807

    PubMed  CAS  Google Scholar 

  • Raudsepp T, Santani A, Wallner B, Kata SR, Ren C, Zhang HB, Womack JE, Skow LC, Chowdhary BP (2004) A detailed physical map of the horse Y chromosome. Proc Natl Acad Sci USA 101:9321–9326

    Article  PubMed  CAS  Google Scholar 

  • Robertson SE, Li Y, Scutt CP, Willis ME, Gilmartin PM (1997) Spatial expression dynamics of Men-9 delineate the third floral whorl in male and female flowers of dioecious Silene latifolia. Plant J 12:155–168

    Article  PubMed  CAS  Google Scholar 

  • Scutt CP, Jenkins T, Furuya M, Gilmartin PM (2002) Male specific genes from dioecious white campion identified by fluorescent differential display. Plant Cell Physiol 43:563–572

    Article  PubMed  CAS  Google Scholar 

  • Scutt CP, Kamisugi Y, Sakai F, Gilmartin PM (1997) Laser isolation of plant sex chromosomes: studies on the DNA composition of the X and Y sex chromosomes of Silene latifolia. Genome 40:705–715

    Article  PubMed  CAS  Google Scholar 

  • Telenius H, Carter NP, Bebb CE, Nordenskjodl M, Ponder BAJ, Tunnacliffe A (1992) Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics 13:718–725

    Article  PubMed  CAS  Google Scholar 

  • Vallejos CE, Sakyiama NS, Chase CD (1992) A molecular marker-based linkage map of Phaseolus vulgaris L. Genetics 131:733–740

    PubMed  CAS  Google Scholar 

  • Vallender EJ, Lahn BT (2004) How mammalian sex chromosomes acquired their peculiar gene content. Bioessays 26:159–169

    Article  PubMed  CAS  Google Scholar 

  • Vyskot B, Hobza R (2004) Gender in plants: sex chromosomes are emerging from the fog. Trends Genet 20:432–438

    Article  PubMed  CAS  Google Scholar 

  • Westergaard M (1958) The mechanism of sex determination in dioecious flowering plants. Adv Genet 9:217–281

    Article  PubMed  CAS  Google Scholar 

  • Yang H, McLeese J, Weisbart M, Dionne J-L, Lemaire I, Aubin RA (1993) Simplified high throughput protocol for Northern hybridization. Nucleic Acids Res 21:3337–3338

    Article  PubMed  CAS  Google Scholar 

  • Zhang YH, Stilio VS, Rehman F, Avery A, Mulcahy D, Kesseli R (1998) Y-chromosome specific markers and the evolution of dioecy in the genus Silene. Genome 41:141–147

    Article  CAS  Google Scholar 

  • Zluvova J, Janousek B, Negrutiu I, Vyskot B (2005) Comparison of the X and Y chromosome organization in Silene latifolia. Genetics 170:1431–1434

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the Grant Agency of the Czech Republic (grants 521/06/0056 and 204/05/H505 to B. V., 521/05/2076 to B. J., and 204/05/P505 to J. Z.) and the institution research plan (A50040507).

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Authors and Affiliations

  1. Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska street 135, 612 65, Brno, Czech Republic

    Roman Hobza, Pavla Hrusakova, Bohuslav Janousek, Jitka Zluvova, Elleni Michu & Boris Vyskot

  2. Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic

    Jan Safar, Jan Bartos & Jaroslav Dolezel

Authors
  1. Roman Hobza
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  2. Pavla Hrusakova
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  3. Jan Safar
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  4. Jan Bartos
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  5. Bohuslav Janousek
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  6. Jitka Zluvova
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  7. Elleni Michu
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  8. Jaroslav Dolezel
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  9. Boris Vyskot
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Correspondence to Boris Vyskot.

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Communicated by A. Kilian

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Hobza, R., Hrusakova, P., Safar, J. et al. MK17, a specific marker closely linked to the gynoecium suppression region on the Y chromosome in Silene latifolia . Theor Appl Genet 113, 280–287 (2006). https://doi.org/10.1007/s00122-006-0293-3

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  • DOI: https://doi.org/10.1007/s00122-006-0293-3

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