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Identification and mapping of cleistogamy genes in barley

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Abstract

Cleistogamy is a closed type of flowering with ensured self-pollination and an important trait to study evolutionary development in flower organs, reproduction systems, gene flow, and disease control. Still, very limited information is available about the genetic control and regulatory mechanism of this trait in barley. In this work, from the eight crosses between cleistogamous and chasmogamous accessions, five crosses generated chasmogamous F1 plants and their F2 plants segregated as 3 chasmogamous:1 cleistogamous, whereas three crosses generated cleistogamous F1 plants, and their F2 plants segregated as 1 chasmogamous:3 cleistogamous. Although a single gene was responsible for the control of cleistogamy in these two groups of crosses, the direction of dominance was opposite, suggesting two genes, cly1 and Cly2, for the genetic control of cleistogamy in barley. Epistatic type of gene interaction between the two loci was detected. In the analysis of 99 recombinant inbred lines of ‘Azumamugi’ × ’Kanto Nakate Gold’ and doubled haploid lines of ‘Harrington’ × ’Mikamo Golden’, where in both crosses F1 was chasmogamous, the cly1 locus has been mapped on chromosome 2HL. Using the analysis of the F2 population of ‘Misato Golden’ and ‘Satsuki Nijo’ where F1 was cleistogamous, the Cly2 locus was mapped in the same region of chromosome 2HL. Because the cly1 and Cly2 loci were mapped in the same region in these three different mapping populations, it was concluded that the expression of cleistogamy is under the control of two tightly linked genes or different alleles of the same gene.

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Acknowledgements

The authors thank Dr. T. Konishi (Okayama, Japan), Dr. K. Takeda (Okayama University, Kurashiki, Japan), and Dr. R. Takahashi (NICS) for reviewing the manuscript and referees for their valuable suggestions. We thank Dr. M. Furusho (Fukuoka Agricultural Research Center, Japan) for help in producing DH lines. The authors acknowledge financial support from the grants provided by Ministry of Agriculture, Forestry and Fisheries of Japan, and CREST, Japan Science and Technology Corporation (JST). Y. Turuspekov is a research fellow supported by Cooperative System for Supporting Priority Research working under the JST.

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Author notes

  1. Y. Mano

    Present address: National Institute of Livestock and Grassland Science, Nishinasuno, 329-2793, Japan

  2. I. Honda

    Present address: National Institute of Vegetables and Tea Science, Ano, 514-2392, Japan

  3. N. Kawada

    Present address: National Agricultural Research Center for Kyushu Okinawa Region, Chikugo, 833-0041, Japan

Authors and Affiliations

  1. National Institute of Crop Science (NICS), Tsukuba, 305-8518, Japan

    Y. Turuspekov, I. Honda, N. Kawada & Y. Watanabe

  2. National Institute of Agrobiological Sciences (NIAS), Tsukuba, 305-8602, Japan

    Y. Mano & T. Komatsuda

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  1. Y. Turuspekov
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Correspondence to T. Komatsuda.

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Communicated by P. Langridge

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Turuspekov, Y., Mano, Y., Honda, I. et al. Identification and mapping of cleistogamy genes in barley. Theor Appl Genet 109, 480–487 (2004). https://doi.org/10.1007/s00122-004-1673-1

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  • DOI: https://doi.org/10.1007/s00122-004-1673-1

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