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Construction of a comparative RFLP map of Echinochloa crus-galli toward QTL analysis of flooding tolerance

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Abstract

To analyze quantitative trait loci (QTLs) affecting flooding tolerance and other physiological and morphological traits in Echinochloa crus-galli, a restriction fragment length polymorphism (RFLP) map was constructed using 55 plants of the F2 population (E. crus-galli var. praticola × E. crus-galli var. formosensis). One hundred forty-one loci formed 41 linkage groups. The total map size was 1,468 cM and the average size of linkage groups was 35.8 cM. The average distance between markers was 14.7 cM and the range was 0–37.2 cM. Early comparisons to the genetic maps of other taxa suggest appreciable synteny with buffelgrass (Pennisetum spp.) and sorghum (Sorghum spp.). One hundred ninty-one F2 plants were used to analyze QTLs of flooding tolerance, plant morphology, heading date, number of leaves, and plant height. For flooding tolerance, two QTLs were detected and one was mapped on linkage group 24. Other traits, including plant morphology, heading date, number of leaves, and plant height were highly correlated. Three genomic regions accounted for most of the mapped QTLs, each explaining 2–4 of the significant marker-trait associations. The high observed correlation between the traits appears to result from QTLs with a large contribution to the phenotypic variance at the same or nearby locations.

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Acknowledgements

This work was supported by the University of Maine Agricultural and Forest Experiment Station (Project No. ME08756-01, External Publication No. 2639, M.E.R.); National Science Foundation (98-72649 and 01-15903, A.H.P.), Texas Agricultural Experiment Station (TEX08346, M.E.R.), Texas Higher Education Coordinating Board (999902-157, M.A.H.), and a Sigma Xi Grant-in-Aid of Research (T.F.).

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Author notes

  1. T. Fukao

    Present address: Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA

Authors and Affiliations

  1. Department of Horticultural Sciences and Program in Molecular and Environmental Plant Sciences, Texas A&M University, College Station, TX 77843, USA

    T. Fukao

  2. Plant Genome Mapping Laboratory, University of Georgia, Athens, GA 30602, USA

    A. H. Paterson

  3. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA

    M. A. Hussey

  4. The Graduate School of Agriculture, Kyoto University, 606–8502, Kyoto, Japan

    Y. Yamasue

  5. Department of Biology, University of Maine, Orono, ME 04469, USA

    R. A. Kennedy

  6. Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, ME 04469, USA

    M. E. Rumpho

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  1. T. Fukao
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  2. A. H. Paterson
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  3. M. A. Hussey
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  4. Y. Yamasue
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  5. R. A. Kennedy
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  6. M. E. Rumpho
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Corresponding author

Correspondence to M. E. Rumpho.

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Communicated by D.J. Mackill

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Fukao, T., Paterson, A.H., Hussey, M.A. et al. Construction of a comparative RFLP map of Echinochloa crus-galli toward QTL analysis of flooding tolerance. Theor Appl Genet 108, 993–1001 (2004). https://doi.org/10.1007/s00122-003-1530-7

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  • DOI: https://doi.org/10.1007/s00122-003-1530-7

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