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Metabolomics study on the synergistic interaction between Salvia miltiorrhiza and Lignum dalbergiae odoriferae used as ‘Jun-Shi’ herbs in a S. miltiorrhiza recipe

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Abstract

A metabolomic method was established to investigate the plasma metabolic difference between healthy rabbits and rabbits with Qi-stagnancy and blood stasis. All rabbits were administrated with an extraction of Salvia miltiorrhiza and S. miltiorrhiza coupled with Lignum dalbergiae odoriferae. The main compounds in plasma samples were detected by high-performance liquid chromatography/diode array detector/electrospray-mass spectrometry (HPLC/DAD/ESI-MS). The data were analyzed by principal component analysis (PCA). The results showed that Qi-stagnancy and blood stasis had a close relationship with dopamine. In addition, the results also indicated that the major plasma metabolic difference between rabbits administrated with S. miltiorrhiza and S. miltiorrhiza coupled with Lignum dalbergiae odoriferae were 4-methylbenzamide, Danshensu and β-(3,4-dihydroxybenzene)-α-hydroxyl propanoic acid isopropyl ester. The above results could provide experimental evidence for the theory of traditional Chinese medicine.

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Acknowledgments

This work was supported by grants from the Ministry of Education of the People’s Republic of China (207151), the Ministry of Science & Technology (2009ZX09103-121) and Chinese Academy of Sciences (2007DF02).

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Authors and Affiliations

  1. College of Life Sciences, Northwest University, 195#, No. 229, Taibai North Road, Xi’an, 710069, China

    Shi-Xiang Wang, Fei Fan & Xiao-Hui Zheng

  2. Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi’an, 710069, China

    Kai Luo, Jing Liang, Hua Li, Jian-Bin Zheng & Xiao-Hui Zheng

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  1. Shi-Xiang Wang
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  2. Kai Luo
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  3. Jing Liang
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  4. Fei Fan
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  5. Hua Li
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  6. Jian-Bin Zheng
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  7. Xiao-Hui Zheng
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Correspondence to Xiao-Hui Zheng.

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Wang, SX., Luo, K., Liang, J. et al. Metabolomics study on the synergistic interaction between Salvia miltiorrhiza and Lignum dalbergiae odoriferae used as ‘Jun-Shi’ herbs in a S. miltiorrhiza recipe. Med Chem Res 20, 16–22 (2011). https://doi.org/10.1007/s00044-009-9275-8

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  • DOI: https://doi.org/10.1007/s00044-009-9275-8

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