Abstract
Adipose tissue is a reliable source of mesenchymal stromal cells (MSC) for use in regenerative medicine. The aim of this pilot study was to describe the method, and assess the safety and the potential efficacy of transplantation of autologous adipose tissue-derived MSC for the treatment of chronic venous stasis ulcers. Study group consisted of 11 patients (mean age: 66.6 ± 9.5 years) with chronic venous stasis ulcers. Adipose tissue was harvested by tumescent-aspiration method. Stromal cells were separated using a dedicated closed system in a real-time bedside manner. The phenotype of cells was determined immediately after separation. Cell concentrate was implanted subcutaneously around the wound and the wound bed. All ulcers were assessed planimetrically before autotransplantation and every two weeks during the six-month follow-up. During the study all patients received standard local and general treatment. The preparation contained an average of 5.6 × 106 ± 4 × 106 cells per milliliter. The phenotype of 65–82% of transplanted cells expressed MSC markers: CD73+ CD90+ and CD34+. An improvement was observed in 75% of ulcers. The data showed highly significant negative correlation (p < 0.0001) between wound size and wound closure degree. There was no correlation of ulcer healing with other parameters evaluated, including age of the patients. No serious side effects were observed. Autotransplantation of adipose tissue stromal cells may be a safe and promising treatment method for chronic venous ulcers.
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Funding
This study was supported by the European Regional Development Fund within the Innovative Economy Operational Program, 2007–2013, project “Wrovasc—Integrated Cardiovascular Centre” (Grant no. POIG.01.01.02-02-001/08.00), realized in the Regional Specialist Hospital, Research and Development Centre in Wroclaw (WW) and by Wroclaw Centre of Biotechnology, National Scientific Leading Centers (KNOW) are the flagship units of Polish science. These research centers receive additional funding for research (Grant no. 2012/07/B/NZ4/01820). This funding sources have no involvement in conducting this study and preparation of the manuscript.
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Masłowski, L., Paprocka, M., Czyżewska-Buczyńska, A. et al. Autotransplantation of the Adipose Tissue-Derived Mesenchymal Stromal Cells in Therapy of Venous Stasis Ulcers. Arch. Immunol. Ther. Exp. 68, 5 (2020). https://doi.org/10.1007/s00005-020-00571-9
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DOI: https://doi.org/10.1007/s00005-020-00571-9