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Effects of diethyl-dithiocarbamate on antioxidant system in carp tissue

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Abstract

The influence the pro- and antioxidant values the diethyl-dithiocarbamate (DDTC) in different concentrations in carp tissues was studied.

From antioxidant enzymes superoxide dismutase, catalase and glutathion peroxidase activity changes were studied in tissue homogenates.

Beside enzyme activities tissue lipid peroxidation (LP), reduced glutathione (GSH) and hydroxyl radical loading of the tissues were measured.

Our results indicate that DDTC affects antioxidant parameters by generating GSH excess, not loading the cells thereby with “oxidative stress”.

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References

  1. Aceto, A., Amicarelli, F., Sachetta, P., Dragani, B., Bucciarelli, T., Masciocco, L., Miranda, M., Di Ilio, C. (1994) Developmental spects of detoxifying enzymes in fish (Salmo irideus). Free Rad. Res. 21, 285–294.

    Article  CAS  Google Scholar 

  2. Beauchamp, C., Fridovich, I. (1971) Superoxide dismutase: improved assay applicable to acrylamide gels. Anal. Biochem. 44, 276–287.

    Article  CAS  Google Scholar 

  3. Beers, R. F. Jr., Sizer, I. W. (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem. 195, 133–140.

    CAS  PubMed  Google Scholar 

  4. Bergmeyer, H. U. (ed.) (1983) Methods of enzymatic analysis. Vol. III. Enzymes 1: Oxido-reductases, transferases. Verlag Chemie, Weinheim.

    Google Scholar 

  5. Cheeseman, K. H., Beavis, A., Esterbauer, H. (1988) Hydroxyl-radical-induced iron-catalysed degradation of 1-deoxyribose. Quantitative determination of malonyl-dialdehyde. Biochem. J. 252, 649–653.

    Article  CAS  Google Scholar 

  6. Chiu, D. T. Y., Stults, F. H., Tappel, A. L. (1976) Purification and properties of rat lung soluble glutathione peroxidase. Biochim. Biophys. Acta 445, 558–566.

    Article  CAS  Google Scholar 

  7. Do Quy Hai, Varga, Sz. I., Matkovics, B. (1995) Organophosphate effects on the antioxidant system of fishes. Acta Biol. Hung. 46, 39–50.

    Google Scholar 

  8. Ellman, G. I., Courtney, K. D., Andres, V. Jr., Featherstone, M. (1961) A new rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7, 88–95.

    Article  CAS  Google Scholar 

  9. Gabryelak, T., Pérés, G. (1986) Comparative antioxidant enzyme and lipid peroxidation study in erythrocytes and liver of some freshwater fish. Acta Biol. Hung. 37, 219–224.

    CAS  PubMed  Google Scholar 

  10. Heikkila, R. E., Cabbat, F. S., Cohen, G. (1976) In vivo inhibition of superoxide dismutase in mice by diethyldithiocarbamate. J. Biol. Chem. 251, 2182–2185.

    CAS  PubMed  Google Scholar 

  11. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. 3. Biol. Chem. 193, 31–34.

    Google Scholar 

  12. Mankhetkorn, S., Abedinzadeh, Z., Houee-Levin, C. (1994) Antioxidant action of sodium diethyldithiocarbamate: Reaction with hydrogen peroxide and superoxide radical. Free Radical Biol. Med. 17, 517–527.

    Article  CAS  Google Scholar 

  13. Matkovics, B., Novák, R., Hoang Duc Hahn, Szabó, L., Varga, Sz. I., Zalesna, G. (1977) A comparative study of some important experimental animal peroxide metabolism enzymes. Comp. Biochem. Physiol. 568, 31–34.

    Google Scholar 

  14. Matkovics, B., Szabó, L., Varga, Sz. I., Barabás, K., Berencsi, G., Nemcsók, J. (1984) Effects of a herbicide on the peroxide metabolism enzymes and lipid peroxidation in carp fish (Hypophthalmichthys molitrix). Acta Biol. Hung. 35, 91–96.

    CAS  PubMed  Google Scholar 

  15. Matkovics, B., Szabó, L., Sz.-né Varga, I. (1988) Determination of enzyme activities, lipid peroxidation and glutathione pathway. Laboratóriumi Diagnosztika 15, 248–250 (in Hungarian with English summary).

    Google Scholar 

  16. Matkovics, B., Sz.-né Varga, I., Novák, Z. (1991) Oxigén gyökök mennyiségi meghatározása biológiai mintákban. Laboratóriumi Diagnosztika 18, 40–46 (in Hungarian with English summary).

    Google Scholar 

  17. Malmström, B. G., Andréasson, L.-E., Reinhammer, B. (1975) Copper-contain oxidases and superoxide dismutase. In: Boyer, P. D. (ed.) The Enzymes. Vol. XIII. pp. 507–579. Academic Press, New York.

    Chapter  Google Scholar 

  18. Mézes, M., Ling, P. (1986) Changes in lipid peroxidation and in the activities of some peroxide metabolism enzymes of blood and certain tissues of common carp during wintering period. Aquaculture Hung. 5, 91–96.

    Google Scholar 

  19. Misra, H. P., Fridovich, I. (1972) The role of superoxide anion in the autoxidation of epinephrine and simple assay for superoxide dismutase. J. Biol. Chem. 247, 3170–3175.

    CAS  PubMed  Google Scholar 

  20. Placer, Z. A., Cushman, L. L., Johnson, B. C. (1966) Estimation of product of lipid peroxidation malonyl dialdehyde in bronchial system. Anal. Biochem. 16, 359–364.

    Article  CAS  Google Scholar 

  21. Rady, A. A. R., Do Qui Hai, Matkovics, B., Gabryelak, T. (1985) Comparative antioxidant enzyme study in freshwater fish with different types of feeding behavior. Comp. Biochem. Physiol. 81C, 395–399.

    Google Scholar 

  22. Sedlak, I., Lindsay, R. H. (1968) Estimation of total protein-bound and nonprotein sulfhydryl groups in the tissue with Ellman’s reagent. Anal. Biochem. 25, 192–205.

    Article  CAS  Google Scholar 

  23. Trombetta, L. D., Toulon, M., Jemall, I. Siraj (1988) Protective effects of glutathione on diethyldithiocarbamate (DDC) cytotoxicity: A possible mechanism. Toxicol. Appl. Pharmacol. 93, 154–164.

    Article  CAS  Google Scholar 

  24. Wimterbourn, C. (1991) Factors influence the deoxyribose oxidation assay for Fenton reaction products. Free Rad. Biol. Med. 11, 353–360.

    Article  Google Scholar 

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Quy Hai, D., Varga, S.I. & Matkovics, B. Effects of diethyl-dithiocarbamate on antioxidant system in carp tissue. BIOLOGIA FUTURA 48, 1–8 (1997). https://doi.org/10.1007/BF03543169

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